1887

Abstract

The taxonomic position of an actinomycete, strain NR4-ASC07, isolated from a soil sample collected from Sirindhorn peat swamp forest, Narathiwat Province, Thailand, was clarified using a polyphasic approach. On the basis of morphological and chemotaxonomic characteristics, it was classified among the members of the genus . It produced tightly closed spiral spore chains on aerial mycelium as well as forming a pseudosporangium. Whole-cell hydrolysates contained -diaminopimelic acid, glucose, ribose, madurose and mannose. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, -phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, unknown ninhydrin-positive phosphoglycolipids and unknown glycolipid. Menaquiones were MK-9(H), MK-9(H), MK-9(H), MK-10(H) and MK-9(H). Predominant cellular fatty acids were iso-C, C 10-methyl, C, Cω8, C 2-OH and iso-C. The phylogenetic tree reconstructed on the basis of 16S rRNA gene sequences showed that the strain fell within the clade containing FMN03, subsp. NBRC 14098 and subsp. NBRC 15903. The DNA–DNA relatedness and phenotypic data supported that strain NR4-ASC07 was clearly distinguished from the closely related species and represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is NR4-ASC07 (=NBRC 112327=TISTR 2465).

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2017-06-01
2024-04-24
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