sp. nov., isolated from activated sludge Free

Abstract

A Gram-stain-negative, facultatively anaerobic bacterium, designated B6, was isolated from activated sludge of a wastewater treatment plant in South Korea. Cells were oxidase- and catalase-positive and non-motile rods producing yellow carotenoid-type pigments. Growth of B6 was observed at 20–40 °C (optimum, 37 °C) and pH 6.6–8.2 (optimum, pH 7.0) and in R2A broth supplemented with 0–1 % (w/v) NaCl (optimum, 0 %). B6 contained iso-C as the major fatty acid. Menaquinone-6 was detected as the sole respiratory quinone. The G+C content of the genomic DNA of B6 was 31.5 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that B6 formed a tight phylogenetic lineage with members of the genus . B6 was most closely related to R2A-16 (99.0 %), NRS1 (98.7 %) and WB5 (97.4 %), but their DNA–DNA relatedness levels were less than 42.0 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that B6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B6 (=KACC 18988=JCM 31714).

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2017-06-01
2024-03-28
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