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Abstract

A novel psychrotolerant bacterium, strain ISLP-3, was isolated from a sample of naturally formed ice sculpture on the shore of Lake Podprudnoye in Antarctica. Cells were motile, stained Gram-positive, non-spore-forming, straight or slightly curved rods with the shape of a baseball bat. The new isolate was facultatively anaerobic and catalase-positive. Growth occurred at 3–35 °C with an optimum at 22–24 °C, 0–2 % (w/v) NaCl with an optimum at 0.3 % and pH 6.2–9.5 with an optimum at pH 7.5. Strain ISLP-3 grew on several carbon sources, with the best growth on cellobiose. The isolate possessed ureolytic activity but growth was inhibited by urea. The strain was sensitive to: ampicillin, gentamycin, kanamycin rifampicin, tetracycline and chloramphenicol. Major fatty acids were: anteiso-C, iso-C, C, C and iso-C. The predominant menaquinone was MK-9(H). The genomic G+C content was 69.5 mol%. The 16S rRNA gene showed 99 % sequence similarity to that of ST-26, but their genes shared ≤91 % sequence similarity, suggesting that this new isolate represents a novel species within the genus . This conclusion was supported by average nucleotide identity, which was ≤91 % to the most closely related strain. The name sp. nov. is proposed for the novel species with the type strain ISLP-3=ATCC TSD-17=DSM 100501=JCM 30887). The complete genome draft sequence of ISLP-3 was deposited under IMG OID 2657245272. Emendments to the descriptions of related taxa have been made based on experimental data from our comparative analysis.

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2017-05-01
2020-01-25
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