RT Journal Article SR Electronic(1) A1 von Neubeck, Mario A1 Huptas, Christopher A1 Glück, Claudia A1 Krewinkel, Manuel A1 Stoeckel, Marina A1 Stressler, Timo A1 Fischer, Lutz A1 Hinrichs, Jörg A1 Scherer, Siegfried A1 Wenning, MareikeYR 2017 T1 Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 6 SP 1656 OP 1664 DO https://doi.org/10.1099/ijsem.0.001836 PB Microbiology Society, SN 1466-5034, AB Five strains, designated WS 4672T, WS 4998, WS 4992T, WS 4997 and WS 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. The most similar species on the basis of 16S rRNA gene sequences were Pseudomonas azotoformans IAM 1603T, Pseudomonas gessardii CIP 105469T and Pseudomonas libanensis CIP 105460T showing 99.7–99.6 % similarity. Using rpoD gene sequences Pseudomonas veronii LMG 17761T (93.3 %) was most closely related to strain WS 4672T and Pseudomonas libanensis CIP 105460T to strain WS 4992T (93.3 %). The five strains could be differentiated from their closest relatives and from each other by phenotypic and chemotaxonomic characterization and ANIb values calculated from draft genome assemblies. ANIb values of strains WS 4992T and WS4671T to the closest relatives are lower than 90 %. The major cellular polar lipids of both strains are phosphatidylethanolamine, phosphatidylglycerol, a phospholipid and diphosphatidylglycerol, and their major quinone is Q-9. The DNA G+C content of strains WS 4992T and WS 4672T were 60.0  and 59.7  mol%, respectively. Based on these genotypic and phenotypic traits two novel species of the genus Pseudomonas are proposed: Pseudomonas lactis sp. nov. [with type strain WS 4992T (=DSM 29167T=LMG 28435T) and the additional strains WS 4997 and WS 5000], and Pseudomonas paralactis sp. nov. [with type strain WS 4672T (=DSM 29164T=LMG 28439T) and additional strain WS 4998]., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001836