sp. nov., isolated from the Asan Bay estuary Free

Abstract

A Gram-stain-negative, strictly aerobic and moderate halotolerant bacterial strain, designated S2-26, was isolated from sediment of the Asan Bay estuary in South Korea. Cells were motile rods with two polar flagella showing oxidase and catalase activities. Growth of S2-26 was observed at 15–45 °C (optimum, 25 °C) and pH 5.5–10.0 (optimum, pH 7.0–8.5) and in the presence of 0–8.0 % (w/v) NaCl (optimum, 2.0 %). S2-26 contained Cω8, summed feature 8 (comprising Cω7 and/or Cω6), summed feature 3 (comprising C 7/C 6) and C as the major fatty acids and ubiquinone-8 as the sole isoprenoid quinone. The polar lipids of S2-26 consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid, an unknown aminolipid, an unknown glycolipid and an unknown lipid. The G+C content of the genomic DNA was 62.2 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that S2-26 formed a tight phylogenetic lineage with 7SM29 with a 100 % bootstrap value. S2-26 was most closely related to the type strain of , with a 97.8 % 16S rRNA gene sequence similarity, and its DNA–DNA relatedness level was 45.2±2.2 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that S2-26 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S2-26 (=KACC 18801=JCM 31547).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001830
2017-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/5/1431.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001830&mimeType=html&fmt=ahah

References

  1. Urios L, Intertaglia L, Lesongeur F, Lebaron P. Haliea salexigens gen. nov., sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 2008; 58:1233–1237 [View Article][PubMed]
    [Google Scholar]
  2. Urios L, Intertaglia L, Lesongeur F, Lebaron P. Haliea rubra sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 2009; 59:1188–1192 [View Article][PubMed]
    [Google Scholar]
  3. Spring S, Riedel T, Spröer C, Yan S, Harder J et al. Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans. BMC Microbiol 2013; 13:118 [View Article][PubMed]
    [Google Scholar]
  4. Lucena T, Pascual J, Garay E, Arahal DR, Macián MC et al. Haliea mediterranea sp. nov., a marine gammaproteobacterium. Int J Syst Evol Microbiol 2010; 60:1844–1848 [View Article][PubMed]
    [Google Scholar]
  5. Lin CY, Zhang XY, Liu A, Liu C, Song XY et al. Haliea atlantica sp. nov., isolated from seawater, transfer of Haliea mediterranea to Parahaliea gen. nov. as Parahaliea mediterranea comb. nov. and emended description of the genus Haliea. Int J Syst Evol Microbiol 2015; 65:3413–3418 [View Article][PubMed]
    [Google Scholar]
  6. Jeong SH, Jin HM, Lee HJ, Jeon CO. Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 2013; 63:971–976 [View Article][PubMed]
    [Google Scholar]
  7. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  8. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007; 3:e56 [View Article][PubMed]
    [Google Scholar]
  9. Felsenstein J. PHYLIP (phylogeny inference package), Version 3.6a. Seattle, WA, USA, Department of GeneticsUniversity of Washington: 2002
    [Google Scholar]
  10. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article]
    [Google Scholar]
  11. Chang HW, Nam YD, Jung MY, Kim KH, Roh SW et al. Statistical superiority of genome-probing microarrays as genomic DNA–DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. J Microbiol Methods 2008; 75:523–530 [View Article][PubMed]
    [Google Scholar]
  12. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PA, Kämpfer P et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002; 52:1043–1047 [View Article][PubMed]
    [Google Scholar]
  13. Gomori G. Preparation of buffers for use in enzyme studies. In Colowick SP, Kaplan NO. (editors) Methods in Enzymology. Academic Press, New York 1955 pp. 138–146
    [Google Scholar]
  14. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  15. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67 [CrossRef]
    [Google Scholar]
  16. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. (editor) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  17. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993
    [Google Scholar]
  18. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–208 [CrossRef]
    [Google Scholar]
  19. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  20. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
    [Google Scholar]
  21. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001830
Loading
/content/journal/ijsem/10.1099/ijsem.0.001830
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed