1887

Abstract

Vernonia brasiliana is a wild perennial shrub frequently found in pasture areas. Plants of this species have been observed displaying typical symptoms induced by phytoplasmas, which were characterized by shoot proliferation, deformed leaves and leaf chlorosis. The present study confirmed the presence of phytoplasmas in association with affected plants. Sequencing of the 16S rRNA gene, computer-simulated RFLP analysis and phylogenetic analysis revealed that one of the phytoplasmas identified was representative of novel subgroup. The sequence identity scores between the novel strain and those of previously described ‘Candidatus Phytoplasma fraxini’-related strains was 99 %, while similarity coefficient values were lower than 0.97. These findings provide support to delineate the phytoplasma found in vernonia plants as a reference phytoplasma for a novel subgroup designated 16SrVII-F. This representative of the novel subgroup was denominated VbSP phytoplasma (Vernonia brasiliana Shoot Proliferation; GenBank KX342018). The results of the present study revealed V. brasiliana to be a host of phytoplasmas, evidenced a novel phytoplasma associated with phytoplasmal disease in Brazil and extended the knowledge of the genetic diversity existing within the 16SrVII group.

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2017-08-09
2019-10-20
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References

  1. Hind DJN. Compositae. In Stannard BL. (editor) Flora of the Pico Das Almas-Chapada Diamantina, Bahia, Brazil Kew: Royal Botanic Gardens; 1995; pp.245–247
    [Google Scholar]
  2. Cronquist A. The Evolution and Classification of Flowering Plants, 2nd ed. Bronx, NY: The New York Botanical Garden; 1988
    [Google Scholar]
  3. Filizola LRS, Pimentel RRM, Randau KP, Xavier HS. Anatomia dos órgãos vegetativos de Vernonia brasiliana (L.) Druce. Lat Am J Pharm 2003;22:299–303
    [Google Scholar]
  4. Wei W, Davis RE, Lee IM, Zhao Y. Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new Phytoplasma groups. Int J Syst Evol Microbiol 2007;57:1855–1867 [CrossRef][PubMed]
    [Google Scholar]
  5. Lee IM, Davis RE, Gundersen-Rindal DE. Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 2000;54:221–255 [CrossRef][PubMed]
    [Google Scholar]
  6. Griffiths HM, Sinclair WA, Smart CD, Davis RE. The phytoplasma associated with ash yellows and lilac witches'-broom: 'Candidatus Phytoplasma fraxini'. Int J Syst Bacteriol 1999;49:1605–1614 [CrossRef][PubMed]
    [Google Scholar]
  7. Zunnoon-Khan S, Arocha-Rosete Y, Scott J, Crosby W, Bertaccini A et al. First report of ‘Candidatus Phytoplasma fraxini’ (group 16SrVII Phytoplasma) associated with a peach disease in Canada. New Disease Reports 2010;21:20
    [Google Scholar]
  8. Gajardo A, Fiore N, Prodan S, Paltrinieri S, Botti S et al. Phytoplasmas associated with grapevine yellows disease in Chile. Plant Disease 2009;93:789–796 [CrossRef]
    [Google Scholar]
  9. Barros TSL, Davis RE, Resende RO, Dally EL. Erigeron witches'-broom phytoplasma in Brazil represents new subgroup VII-B in 16S rRNA gene group VII, the Ash Yellows phytoplasma group. Plant Disease 2002;86:1142–1148 [CrossRef]
    [Google Scholar]
  10. Meneguzzi NG, Torres LE, Galdeano E, Guzman FA, Nome SF et al. Molecular characterization of a phytoplasma of the Ash Yellows group (16Sr VII-B) occurring in Artemisia annua and Conyza bonariensis weeds. Agriscientia 2008;52:7–15
    [Google Scholar]
  11. Conci L, Meneguzzi N, Galdeano E, Torres L, Nome C et al. Detection and molecular characterisation of an alfalfa phytoplasma in Argentina that represents a new subgroup in the 16S rDNA Ash Yellows group (‘Candidatus Phytoplasma fraxini’). Eur J Plant Pathol 2005;113:255–265 [CrossRef]
    [Google Scholar]
  12. Flôres D, Mello A, Massola Júnior NS, Bedendo IP. First report of a group 16SrVII-C phytoplasma associated with shoot proliferation of sunn hemp in Brazil. Plant Dis 2013;97:1652[CrossRef]
    [Google Scholar]
  13. Flôres D, Amaral Mello AP, Pereira TB, Rezende JA, Bedendo IP. A novel subgroup 16SrVII-D phytoplasma identified in association with erigeron witches' broom. Int J Syst Evol Microbiol 2015;65:2761–2765 [CrossRef][PubMed]
    [Google Scholar]
  14. Pérez-López E, Luna-Rodríguez M, Olivier CY, Dumonceaux TJ. The underestimated diversity of phytoplasmas in Latin America. Int J Syst Evol Microbiol 2016;66:492–513 [CrossRef][PubMed]
    [Google Scholar]
  15. James AP, Geijskes RJ, Dale JL, Harding RM. Development of a novel rolling-circle amplification technique to detect Banana streak virus that also discriminates between integrated and episomal virus sequences. Plant Dis 2011;95:57–62 [CrossRef]
    [Google Scholar]
  16. Gundersen DE, Lee IM. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr 1996;35:144–151
    [Google Scholar]
  17. Malembic-Maher S, Constable F, Cimerman A, Arnaud G, Carle P et al. A chromosome map of the Flavescence doree phytoplasma. Microbiology 2008;154:1454–1463 [CrossRef][PubMed]
    [Google Scholar]
  18. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95–98
    [Google Scholar]
  19. Wei W, Lee IM, Davis RE, Suo X, Zhao Y. Automated RFLP pattern comparison and similarity coefficient calculation for rapid delineation of new and distinct phytoplasma 16Sr subgroup lineages. Int J Syst Evol Microbiol 2008;58:2368–2377 [CrossRef][PubMed]
    [Google Scholar]
  20. Lee I-M, Gundersen-Rindal DE, Davis RE, Bartoszyk IM. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 1998;48:1153–1169 [CrossRef]
    [Google Scholar]
  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  22. Kitajima EW, Nome CF. Microscopia eletronica en virologia vegetal. In Docampo D, Lenardon SL. (editors) Métodos Para Detector Patógenos Sistémicos Córdoba, Argentina: IFFIVE/JICA; 1999; pp.59–87
    [Google Scholar]
  23. Kitajima EW, Leite B. Curso Introdutório De Microscopia Eletrônica De Varredura Piracicaba, Brazil: NAP/MEPA ESALQ; 1998
    [Google Scholar]
  24. Zhao Y, Davis RE, Wei W, Lee IM. Should 'Candidatus Phytoplasma' be retained within the order Acholeplasmatales?. Int J Syst Evol Microbiol 2015;65:1075–1082 [CrossRef][PubMed]
    [Google Scholar]
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