1887

Abstract

Nine novel Gram-stain-positive bacteria were investigated by a polyphasic taxonomic approach. Based on the analysis of 16S rRNA gene sequences, these strains belonged to the Bacillus cereus group, sharing over 97 % similarity with the known species of this group, and less than 95 % similarity with other species of the genus Bacillus . Multilocus sequence typing analysis showed that they formed nine robust and well-separated branches from the known species. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the nine strains were, respectively, below the 70 and 96 % threshold values for species definition, and between each strain and the known type strains of this group were also below the two threshold values. On the basis of the phenotypic and phylogenetic data, along with low dDDH and ANI values among these strains, these bacteria are assigned to the following nine novel species of the B. cereus group: Bacillus paranthracis sp. nov., type strain Mn5 (=MCCC 1A00395=KCTC 33714=LMG 28873); Bacillus pacificus sp. nov., type strain EB422 (=MCCC 1A06182=KCTC 33858); Bacillus tropicus sp. nov., type strain N24 (=MCCC 1A01406=KCTC 33711=LMG 28874); Bacillus albus sp. nov., type strain N35-10-2 (=MCCC 1A02146=KCTC 33710=LMG 28875); Bacillus mobilis sp. nov., type strain 0711P9-1 (=MCCC 1A05942=KCTC 33717=LMG 28877); Bacillus luti sp. nov., type strain TD41 (=MCCC 1A00359=KCTC 33716=LMG 28872); Bacillus proteolyticus sp. nov., type strain TD42 (=MCCC 1A00365=KCTC 33715=LMG 28870); Bacillus nitratireducens sp. nov., type strain 4049 (=MCCC 1A00732=KCTC 33713=LMG 28871); and Bacillus paramycoides sp. nov., type strain NH24A2 (=MCCC 1A04098=KCTC 33709=LMG 28876).

Erratum
This article contains a correction applying to the following content:
Corrigendum: Proposal of nine novel species of the Bacillus cereus group
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001821
2017-08-10
2019-08-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/8/2499.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001821&mimeType=html&fmt=ahah

References

  1. Liu Y, Lai Q, Göker M, Meier-Kolthoff JP, Wang M et al. Genomic insights into the taxonomic status of the Bacillus cereus group. Sci Rep 2015;5:14082 [CrossRef][PubMed]
    [Google Scholar]
  2. Miller RA, Beno SM, Kent DJ, Carroll LM, Martin NH et al. Bacillus wiedmannii sp. nov. is a new psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and environments in the USA. Int J Syst Evol Microbiol 2016;66:4744–4753 [CrossRef][PubMed]
    [Google Scholar]
  3. Rasko DA, Altherr MR, Han CS, Ravel J. Genomics of the Bacillus cereus group of organisms. FEMS Microbiol Rev 2005;29:303–329 [CrossRef][PubMed]
    [Google Scholar]
  4. Liu Y, Lai Q, du J, Shao Z. Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments. Sci Rep 2017;7:689 [CrossRef][PubMed]
    [Google Scholar]
  5. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  6. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  7. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  8. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef]
    [Google Scholar]
  9. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  10. Sullivan CB, Diggle MA, Clarke SC. Multilocus sequence typing: data analysis in clinical microbiology and public health. Mol Biotechnol 2005;29:245–254 [CrossRef][PubMed]
    [Google Scholar]
  11. Priest FG, Barker M, Baillie LW, Holmes EC, Maiden MC. Population structure and evolution of the Bacillus cereus group. J Bacteriol 2004;186:7959–7970 [CrossRef][PubMed]
    [Google Scholar]
  12. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  13. Wayne LG, Brenner D, Colwell R, Grimont P, Kandler O et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  14. Guinebretière MH, Auger S, Galleron N, Contzen M, de Sarrau B et al. Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus group occasionally associated with food poisoning. Int J Syst Evol Microbiol 2013;63:31–40 [CrossRef][PubMed]
    [Google Scholar]
  15. Rainey FA, Whitman WB, Krieg NR, Garrity GM, Vos P et al. Bergey's manual of systematic bacteriology. In The Firmicutesvol. 3 New York: Springer; 2009
    [Google Scholar]
  16. Jiménez G, Urdiain M, Cifuentes A, López-López A, Blanch AR et al. Description of Bacillus toyonensis sp. nov., a novel species of the Bacillus cereus group, and pairwise genome comparisons of the species of the group by means of ANI calculations. Syst Appl Microbiol 2013;36:383–391 [CrossRef][PubMed]
    [Google Scholar]
  17. Liu B, Liu GH, Hu GP, Sengonca C, Cetin S et al. Bacillus bingmayongensis sp. nov., isolated from the pit soil of Emperor Qin's Terra-cotta warriors in China. Antonie van Leeuwenhoek 2014;105:501–510 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001821
Loading
/content/journal/ijsem/10.1099/ijsem.0.001821
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error