1887

Abstract

A novel, thermophilic filamentous anoxygenic phototrophic bacterium, strain isl-2, was isolated from the Strokkur Geyser, Iceland. Strain isl-2 formed unbranched multicellular filaments with gliding motility. The cells formed no spores and stained Gram-negative. The existence of pili was described in a species of the genus Chloroflexus for the first time, to our knowledge. Optimal growth occurred at a pH range of 7.5–7.7 and at a temperature of 55 °C. Strain isl-2 grew photoheterotrophically under anaerobic conditions in the light and chemoheterotrophically under aerobic conditions in the dark. The major cellular fatty acids were C18 : 1ω9, C16 : 0, C18 : 0 and C18 : 0-OH. The major quinone was menaquinone-10. The photosynthetic pigments were bacteriochlorophylls c and a as well as β- and γ-carotenes. The results of phylogenetic analysis of the 16S rRNA gene sequences placed strain isl-2 into the genus Chloroflexus of the phylum Chloroflexi with Chloroflexus aggregans DSM 9485 as the closest relative (97.0 % identity). The whole-genome sequence of isl-2 was determined. Average nucleotide identity values obtained for isl-2 in comparison to available genomic sequences of other strains of members of the genus Chloroflexus were 81.4 % or less and digital DNA–DNA hybridisation values 22.8 % or less. The results of additional phylogenetic analysis of the PufLM and BchG amino acid sequences supported the separate position of the isl-2 phylotype from the phylotypes of other members of the genus Chloroflexus . On the basis of physiological and phylogenetic data as well as genomic data, it was suggested that isl-2 represents a novel species within the genus Chloroflexus , with the proposed name Chloroflexus islandicus sp. nov. The type strain of the species is isl-2 (=VKM B-2978,=DSM 29225,=JCM 30533).

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2017-05-30
2019-10-14
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