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Volume 67, Issue 5

sp. nov., a radiation-resistant bacterium isolated from the Han River Free

Abstract

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-12, was isolated from a water sample after exposure to 3 kGy of gamma radiation. The strain showed resistance to gamma radiation with a dose required to reduce the bacterial population 10 fold (D) value of 4.7 kGy. The results of comparative 16S rRNA gene sequence analysis indicated that strain 15J8-12 represented a member of the family , phylum , and was most closely related to ‘ ’ 15J17 (97.92 %) and R2-35 (92.22 %). The G+C content of the genomic DNA of 15J8-12 was 51.3 mol%. The detection of menaquinone MK-7 as the predominant respiratory quinone, a fatty acid profile with summed feature 3 (Cω7/C ω6; 40.5 %), Cω (35.3 %), C iso (6.9 %) and C (6.8 %) as the major components and phosphatidylethanolamine as the major polar lipid also supported the affiliation of 15J8-12 with the genus . The DNA–DNA relatedness between 15J8-12 and ‘’ 15J17 was 27.8 %. On the basis of its phenotypic and genotypic properties, together with its phylogenetic distinctiveness, 15J8-12 should be considered to be a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 15J8-12 (=KCTC 52510=JCM 31407).

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  • Accepted:
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Keyword(s): Bacteroidetes , polyphasic taxonomy and Spirosoma
© 2017 IUMS
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2017-05-01
2024-04-19
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Spirosoma knui sp. nov., a radiation-resistant bacterium isolated from the Han River
Int J Syst Evol Microbiol 67, 1359 (2017); https://doi.org/10.1099/ijsem.0.001817
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