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Volume 67, Issue 5

gen. nov., sp. nov., a sulfur oxidizer isolated from a hot spring microbial mat Free

Abstract

A novel sulfur-oxidizing autotrophic bacterium, strain J1A was isolated from a hot spring microbial mat. The cells were Gram-stain-negative, catalase-negative and oxidase-positive. As sole electron donor for chemolithoautotrophic growth, strain J1A utilized sulfide, thiosulfate, elemental sulfur, and tetrathionate. The G+C content of the genomic DNA was 66 mol%. Major cellular fatty acids (>40 % of total) were C and summed feature 3 (Cω7 and/or Cω6). The predominant quinone was Q-8. Phylogenetic analysis of the 16S rRNA gene indicated that strain J1A is a relative of species of the genus , but shares only 93 % or lower sequence similarities with them. On the basis of its properties, strain J1A represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is J1A (=DSM 103923=NBRC 112474).

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  • Accepted:
  • Published Online:
Keyword(s): hot spring , sulfur-oxidizing bacteria and Thiobacillus
© 2017 IUMS
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2017-05-01
2023-06-02
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References

  1. Kelly DP, Wood AP, Stackebrandt E. Genus II. Thiobacillus Beijerinck 1904b 597AL. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2, part C New York: Springer; 2005 pp. 764–771 [CrossRef]
     [Google Scholar]
  2. Orlygsson J, Kristjansson JK. The family Hydrogenophilaceae. In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F. (editors) The Prokaryotes-Alphaproteobacteria and Betaproteobacteria, 4th ed. Berlin: Springer; 2014 pp. 859–868 [CrossRef]
     [Google Scholar]
  3. Garrity GM, Bell JA, Lilburn T. Order II. Hydrogenophilales ord. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005a p 763
     [Google Scholar]
  4. Garrity GM, Bell JA, Lilburn T. Family I. Hydrogenophilaceae fam. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005b p 763
     [Google Scholar]
  5. Kojima H, Fukui M. Sulfuritalea hydrogenivorans gen. nov., sp. nov., a facultative autotroph isolated from a freshwater lake. Int J Syst Evol Microbiol 2011; 61:1651–1655 [View Article][PubMed]
     [Google Scholar]
  6. Widdel F, Bak F. Gram-negative mesotrophic sulfate-reducing bacteria. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H. (editors) The Prokaryotes, 2nd ed. vol. 4 New York: Springer-Verlag; 1992 pp. 3352–3378 [CrossRef]
     [Google Scholar]
  7. Kojima H, Watanabe T, Fukui M. Sulfuricaulis limicola gen. nov., sp. nov., a sulfur oxidizer isolated from a lake. Int J Syst Evol Microbiol 2016; 66:266–270 [View Article][PubMed]
     [Google Scholar]
  8. Katayama-Fujimura Y, Komatsu Y, Kuraishi H, Kaneko T. Estimation of DNA base composition by high performance liquid chromatography of its nuclease PI hydrolysate. Agric Biol Chem 1984; 48:3169–3172 [View Article]
     [Google Scholar]
  9. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [View Article][PubMed]
     [Google Scholar]
  10. Kojima H, Shinohara A, Fukui M. Sulfurifustis variabilis gen. nov., sp. nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov. Int J Syst Evol Microbiol 2015; 65:3709–3713 [View Article][PubMed]
     [Google Scholar]
  11. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chapter 6 Chichester: Wiley; 1991 pp. 115–175
     [Google Scholar]
  12. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
     [Google Scholar]
  13. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  14. Gray CJ, Engel AS. Microbial diversity and impact on carbonate geochemistry across a changing geochemical gradient in a karst aquifer. Isme J 2013; 7:325–337 [View Article][PubMed]
     [Google Scholar]
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Sulfuritortus calidifontis gen. nov., sp. nov., a sulfur oxidizer isolated from a hot spring microbial mat
Int J Syst Evol Microbiol 67, 1355 (2017); https://doi.org/10.1099/ijsem.0.001813
/content/journal/ijsem/10.1099/ijsem.0.001813
/content/journal/ijsem/10.1099/ijsem.0.001813
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