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Volume 67, Issue 5

sp. nov., a spirochaete isolated from the bovine rumen Free

Abstract

A novel bacterium, strain Ru1, was encountered during a survey of spirochaetes living in the gastrointestinal tract of ruminants. Comparative analysis of 16S rRNA gene sequence data indicated that strain Ru1 clustered within the genus but shared at most 86.1 % sequence similarity with other recognised species of the genus . Further phylogenetic analysis based on partial recombinase A () gene sequence comparisons, together with phenotypic characterization, also demonstrated the divergence of strain Ru1 from other recognised species of the genus . Microscopically, strain Ru1 appeared as a very small, highly motile, helical spirochaete with eight periplasmic flagella in a 4 : 8 : 4 arrangement. It exhibited C8 esterase lipase, leucine arylamidase, β-galactosidase and β-glucosidase activity. A distinctive, serum-independent growth pattern was also observed, characterized by colonies with an absence of the local haemolysis that is typical of many pathogenic treponemes. On the basis of these data, strain Ru1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Ru1 (=DSM 103462=NCTC 13847).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bovine , Rumen , Spirochaete and Treponema
© 2017 IUMS
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2017-05-01
2024-04-24
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Treponema ruminis sp. nov., a spirochaete isolated from the bovine rumen
Int J Syst Evol Microbiol 67, 1349 (2017); https://doi.org/10.1099/ijsem.0.001812
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