1887

Abstract

A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13 were non-motile, rod-shaped and 3–10 µm in length. The optimum growth conditions were 3.5–5.0 M NaCl, 45–55 °C, and pH range of 6.5–8.2. The major polar lipids were CC and CC derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13 was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and genes revealed that strain H13 was most closely related to AB14 (16S rRNA gene sequence similarity: 99.51 %; sequence similarity: 96.19 %) and PR5 (99.11 %; 95.50 %). Strain H13 showed low genome relatedness values with AB14 and PR5 based on estimated average nucleotide identity (ANI; 92.59 and 91.68 %, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00 %, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H13 (=BCRC 910151=NBRC 111885).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001811
2017-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/5/1333.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001811&mimeType=html&fmt=ahah

References

  1. Grant WD, Kamekura M, McGenity TJ, Ventosa A. Order I. Halobacteriales Grant and Larsen 1989b, 495VP (Effective publication: Grant and Larsen 1989a, 2216). In Boone DR, Castenholz RW, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 1 New York: Springer; 2001 pp. 294–299
    [Google Scholar]
  2. Oren A. The order Halobacteriales. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E. et al. (editors) The Prokaryotes. an Evolving Electronic Resource for the Microbiological Community, Release 3.2 New York: Springer; 2000
    [Google Scholar]
  3. Ventosa A. Unusual micro-organisms from unusual habitats: hypersaline environments. In Logan NA, Lappin-Scott HM, Oyston PCF. (editors) Prokaryotic Diversity: Mechanisms and Significance (Society for General Microbiology Symposium No. 66) Cambridge: Cambridge University Press; 2006 pp. 223–253 [CrossRef]
    [Google Scholar]
  4. Ventosa A, Gutiérrez MC, Kamekura M, Dyall-Smith ML. Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 1999; 49:131–136 [View Article][PubMed]
    [Google Scholar]
  5. Montalvo-Rodríguez R, López-Garriga J, Vreeland RH, Oren A, Ventosa A et al. Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico. Int J Syst Evol Microbiol 2000; 50:1065–1071 [View Article][PubMed]
    [Google Scholar]
  6. Xu XW, Liu SJ, Tohty D, Oren A, Wu M et al. Haloterrigena saccharevitans sp. nov., an extremely halophilic archaeon from Xin-Jiang, China. Int J Syst Evol Microbiol 2005; 55:2539–2542 [View Article][PubMed]
    [Google Scholar]
  7. Cui HL, Tohty D, Zhou PJ, Liu SJ. Haloterrigena longa sp. nov. and Haloterrigena limicola sp. nov., extremely halophilic archaea isolated from a salt lake. Int J Syst Evol Microbiol 2006; 56:1837–1840 [View Article][PubMed]
    [Google Scholar]
  8. Romano I, Poli A, Finore I, Huertas FJ, Gambacorta A et al. Haloterrigena hispanica sp. nov., an extremely halophilic archaeon from Fuente De Piedra, Southern Spain. Int J Syst Evol Microbiol 2007; 57:1499–1503 [View Article][PubMed]
    [Google Scholar]
  9. Gutiérrez MC, Castillo AM, Kamekura M, Ventosa A. Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake. Int J Syst Evol Microbiol 2008; 58:2880–2884 [View Article][PubMed]
    [Google Scholar]
  10. Roh SW, Nam YD, Chang HW, Kim KH, Sung Y et al. Haloterrigena jeotgali sp. nov., an extremely halophilic archaeon from salt- fermented food. Int J Syst Evol Microbiol 2009; 59:2359–2363 [View Article][PubMed]
    [Google Scholar]
  11. Wang S, Yang Q, Liu ZH, Sun L, Wei D et al. Haloterrigena daqingensis sp. nov., an extremely haloalkaliphilic archaeon isolated from a saline-alkaline soil. Int J Syst Evol Microbiol 2010; 60:2267–2271 [View Article][PubMed]
    [Google Scholar]
  12. Jarrell KF, Faguy D, Hebert AM, Kalmokoff ML. A general method of isolating high molecular weight DNA from Methanogenic archaea (archaebacteria). Can J Microbiol 1992; 38:65–68 [View Article][PubMed]
    [Google Scholar]
  13. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  14. Suyama M, Torrents D, Bork P. PAL2NAL: robust conversion of protein sequence alignments into the corresponding codon alignments. Nucleic Acids Res 2006; 34:W609–W612 [View Article][PubMed]
    [Google Scholar]
  15. Minegishi H, Kamekura M, Itoh T, Echigo A, Usami R et al. Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B' (rpoB') gene. Int J Syst Evol Microbiol 2010; 60:2398–2408 [View Article][PubMed]
    [Google Scholar]
  16. Oren A, Ventosa A, Grant WD. Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 1997; 47:233–238 [View Article]
    [Google Scholar]
  17. Gerhardt P, Murray RGE, Wood WA, Krieg NR. Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994
    [Google Scholar]
  18. Kates M, Kushwaha SC. Isoprenoids and polar lipids of extreme halophiles. In DasSarma S, Fleischmann EM. (editors) Archaea – A Laboratory Manual, Halophiles New York, USA: Cold Spring Harbor Laboratory Press; 1995 pp. 35–57
    [Google Scholar]
  19. Kates M. Techniques of Lipidology New York: North Holland/American Elsevier; 1972 [CrossRef]
    [Google Scholar]
  20. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
    [Google Scholar]
  21. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
    [Google Scholar]
  22. Lai MC, Chen SC. Methanofollis aquaemaris sp. nov., a methanogen isolated from an aquaculture fish pond. Int J Syst Evol Microbiol 2001; 51:1873–1880 [View Article][PubMed]
    [Google Scholar]
  23. Lai MC, Xu SH. Novel polyhydroxyalkanoate by extreme halophilic archaea; 2004 Patent number in Republic of China: 579390
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001811
Loading
/content/journal/ijsem/10.1099/ijsem.0.001811
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error