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Abstract

A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13 were non-motile, rod-shaped and 3–10 µm in length. The optimum growth conditions were 3.5–5.0 M NaCl, 45–55 °C, and pH range of 6.5–8.2. The major polar lipids were CC and CC derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13 was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and genes revealed that strain H13 was most closely related to AB14 (16S rRNA gene sequence similarity: 99.51 %; sequence similarity: 96.19 %) and PR5 (99.11 %; 95.50 %). Strain H13 showed low genome relatedness values with AB14 and PR5 based on estimated average nucleotide identity (ANI; 92.59 and 91.68 %, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00 %, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H13 (=BCRC 910151=NBRC 111885).

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2017-05-01
2020-01-26
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