1887

Abstract

A Gram-strain-negative, rod-shaped, motile bacterium, designated MIM27, was isolated from the sand of the Mu Us Desert, PR China. The strain could grow at 4–45 °C (optimum, 37 °C), at pH 6.6–9.0 (optimum, 8.0) and in the presence of 0–3 % (w/v) NaCl (optimum, 0 % in RNA liquid medium). The results of phylogenetic analysis of 16S rRNA gene sequences indicated that the strain represented a member of the genus , with the highest similarity (96.5 %) to BN-19. The results of analysis of the sequences of the nitrogen fixation gene and three housekeeping genes, , and , also indicated that MIM27 was most closely related to the species of the genus with validly published names but the similarities were low (≤90.7 %). MIM27 did not form nodules on , , and . The major respiratory quinone of MIM27 was Q-10. The genomic DNA G+C content was 59.8 mol%. Major fatty acids of MIM27 were summed feature 8 (Cω7 and/or Cω6), Cω7 11-methyl, C, summed feature 3 (Cω7 and/or Cω6) and summed feature 2 (C aldehyde and/or unknown ECL 10.9525). On the basis of the physiological, chemotaxonomic and phenotypic data, MIM27 is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MIM27 (=KCTC 52299=MCCC 1K03215).

Keyword(s): nifH , nodulation , rhizobium and sand soil
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2017-07-01
2020-01-28
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