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Abstract

Strain NH1, a pink-pigmented, facultatively anaerobic, heterotrophic, catalase-positive and oxidase-negative, Gram-stain-negative marine bacterium, was isolated from marine sediment on the coast of Weihai, China. Cells of strain NH1 were rod-shaped, 0.8–2.0 µm in length and 0.5–1.0 µm in width. The strain was able to grow at 13–37 °C, pH 5.5–8.5, in the presence of 0.0–8.0 % (w/v) NaCl. Optimal growth was observed at 28 °C, with 3.0 % (w/v) NaCl and pH 6.5–7.0. Nitrate was reduced. The G+C content of the DNA was 41.9 mol%. The major isoprenoid quinone was MK-7 and the main cellular fatty acids (>10 %) were summed feature 3 (33.6 %) comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c, and iso-C15:0 (19.2%). The major polar lipids in strain NH1 were phosphatidylethanolamine, unidentified lipids, phospholipid and aminolipids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NH1 was highly related to the type strains of Algoriphagus antarcticus (97.87 % 16SrRNA gene sequence similarity) and Algoriphagus ratkowskyi (97.56 %). On basis of the phenotypic and phylogenetic data, strain NH1 should be classified as representing a novel species of the genus Algoriphagus , for which the name Algoriphagus resistens sp. nov. is proposed. The type strain is NH1 (=MCCC 1H00140=KCTC 52228).

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2017-05-30
2019-09-21
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References

  1. Bowman JP, Nichols CM, Gibson JA. Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 2003;53:1343–1355 [CrossRef][PubMed]
    [Google Scholar]
  2. Park S, Kang SJ, Oh KH, Oh TK, Yoon JH. Algoriphagus lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2010;60:200–204 [CrossRef][PubMed]
    [Google Scholar]
  3. Kang H, Weerawongwiwat V, Jung MY, Myung SC, Kim W. Algoriphagus chungangensis sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2013;63:648–653 [CrossRef][PubMed]
    [Google Scholar]
  4. Park S, Park JM, Lee KC, Yoon JH. Algoriphagus boseongensis sp. nov., a member of the family Cyclobacteriaceae isolated from a tidal flat. Antonie van Leeuwenhoek 2014;105:523–531 [CrossRef][PubMed]
    [Google Scholar]
  5. Kim H, Joung Y, Joh K. Algoriphagus taeanensis sp. nov., isolated from a tidal flat, and emended description of Algoriphagus hitonicola. Int J Syst Evol Microbiol 2014;64:21–26 [CrossRef][PubMed]
    [Google Scholar]
  6. Gerhardt P, Wood WA, Krieg NR. Methods for General and Molecular Bacteriology Washington, DC: ASM Press; 1994
    [Google Scholar]
  7. Barrow G, Cowan FR. Steel's Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press; 1993;[CrossRef]
    [Google Scholar]
  8. Dong X, Cai M. Manual of General Bacteria Systemic Identification Beijing: Science Press; 2001
    [Google Scholar]
  9. Tindall B, Sikorski J, Smibert R, Krieg N. Phenotypic characterization and the principles of comparative systematics. Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007; pp.330–393
    [Google Scholar]
  10. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982;5:2359–2367 [CrossRef]
    [Google Scholar]
  11. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  12. Lee I, Ouk Kim Y, Chun J, Park S-C. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016;66:1100–1103 [CrossRef]
    [Google Scholar]
  13. Liu QQ, Wang Y, Li J, du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014;64:2204–2209 [CrossRef][PubMed]
    [Google Scholar]
  14. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 1999; pp.95–98
    [Google Scholar]
  15. Thompson J, Higgins D, Gibson T. CLUSTAL X multiple sequence alignment program. European Molecular Biology Organization Hamburg, Germany: 1997
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  17. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  18. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 1971;20:406–416 [CrossRef]
    [Google Scholar]
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  20. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef]
    [Google Scholar]
  22. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  23. van Trappen S, Vandecandelaere I, Mergaert J, Swings J. Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 2004;54:1969–1973 [CrossRef][PubMed]
    [Google Scholar]
  24. Chun J, Lee JH, Jung Y, Kim M, Kim S et al. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 2007;57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  25. Yoon JH, Kang SJ, Oh TK. Algoriphagus locisalis sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 2005;55:1635–1639 [CrossRef][PubMed]
    [Google Scholar]
  26. Nedashkovskaya OI, Vancanneyt M, van Trappen S, Vandemeulebroecke K, Lysenko AM et al. Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. Int J Syst Evol Microbiol 2004;54:1757–1764 [CrossRef][PubMed]
    [Google Scholar]
  27. Inan K, Kacagan M, Ozer A, Osman Belduz A, Canakci S. Algoriphagus trabzonensis sp. nov., isolated from freshwater, and emended description of Algoriphagus alkaliphilus. Int J Syst Evol Microbiol 2015;65:2234–2240 [CrossRef][PubMed]
    [Google Scholar]
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