1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 4

sp. nov., isolated from Arctic seawater Free

Abstract

A Gram-reaction-negative, aerobic, non-flagellated, rod-shaped and yellow-pigmented bacterium, designated strain SM1502, was isolated from Arctic seawater. The isolate grew at 10–40 °C and with 0–8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was affiliated with the genus , with the highest sequence similarity (96.0 %) found with XIN-1. The major fatty acids of strain SM1502 were iso-C, iso-Cω9, iso-C G, C, iso-C 3-OH and unknown ECL 13.565. The major respiratory quinone of strain SM1502 was menaquinone-6 (MK-6). Polar lipids of strain SM1502 included phosphatidylethanolamine and one unidentified aminolipid and lipid. The genomic DNA G+C content of strain SM1502 was 37.0 mol%. Based on the polyphasic data obtained in this study, strain SM1502 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SM1502 (=KCTC 42668=CCTCC AB 2015346).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Arctic seawater , sp. nov. and The genus Flavobacterium
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001804
2017-04-01
2023-06-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/4/1070.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001804&mimeType=html&fmt=ahah

References

  1. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FM et al. Genus II. Flavobacterium gen. nov. In Bergey’s Manual of Determinative Bacteriology, 1st ed. Baltimore, MD: Williams & Wilkins; 1923 pp. 97–117
     [Google Scholar]
  2. Bernardet J-F, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 1996; 46:128–148 [View Article]
     [Google Scholar]
  3. Dong K, Chen F, du Y, Wang G. Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense. Int J Syst Evol Microbiol 2013; 63:886–892 [View Article][PubMed]
     [Google Scholar]
  4. Kang JY, Chun J, Jahng KY. Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium. Int J Syst Evol Microbiol 2013; 63:1633–1638 [View Article][PubMed]
     [Google Scholar]
  5. Bernardet J-F, Bowman JP. The genus Flavobacterium. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E. (editors) The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd ed. vol. 7 New York, NY: Springer; 2006 pp. 481–531
     [Google Scholar]
  6. Bernardet J-F, Bowman JP. Genus I. Flavobacterium Bergey, Harrison, Breed, Hammer and Huntoon 1923, 97AL emend. Bernardet, segers, vancanneyt, Berthe, Kersters and Vandamme 1996, 139. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ. et al. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 4 New York, NY: Springer; 2011 pp. 112–154
     [Google Scholar]
  7. Miyashita M, Fujimura S, Nakagawa Y, Nishizawa M, Tomizuka N et al. Flavobacterium algicola sp. nov., isolated from marine algae. Int J Syst Evol Microbiol 2010; 60:344–348 [View Article][PubMed]
     [Google Scholar]
  8. Hu G, Zhang J, Yang G, Li YY, Guan YT et al. Flavobacterium yanchengense sp. nov., isolated from soil. Int J Syst Evol Microbiol 2013; 63:2848–2852 [View Article][PubMed]
     [Google Scholar]
  9. Mccammon SA, Innes BH, Bowman JP, Franzmann PD, Dobson SJ et al. Flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater Antarctic lake. Int J Syst Bacteriol 1998; 48 Pt 4:1405–1412 [View Article][PubMed]
     [Google Scholar]
  10. Mccammon SA, Bowman JP. Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 2000; 50:1055–1063 [View Article][PubMed]
     [Google Scholar]
  11. Humphry DR, George A, Black GW, Cummings SP. Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. Int J Syst Evol Microbiol 2001; 51:1235–1243 [View Article][PubMed]
     [Google Scholar]
  12. van Trappen S, Mergaert J, Swings J. Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 2003; 53:1241–1245 [View Article][PubMed]
     [Google Scholar]
  13. van Trappen S, Vandecandelaere I, Mergaert J, Swings J. Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 2004; 54:85–92 [View Article][PubMed]
     [Google Scholar]
  14. van Trappen S, Vandecandelaere I, Mergaert J, Swings J. Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 2005; 55:769–772 [View Article][PubMed]
     [Google Scholar]
  15. Yi H, Oh HM, Lee JH, Kim SJ, Chun J. Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 2005; 55:637–641 [View Article][PubMed]
     [Google Scholar]
  16. Nogi Y, Soda K, Oikawa T. Flavobacterium frigidimaris sp. nov., isolated from Antarctic seawater. Syst Appl Microbiol 2005; 28:310–315 [View Article][PubMed]
     [Google Scholar]
  17. Yi H, Chun J. Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. Int J Syst Evol Microbiol 2006; 56:1239–1244 [View Article][PubMed]
     [Google Scholar]
  18. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp. 115–175
     [Google Scholar]
  19. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  20. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article][PubMed]
     [Google Scholar]
  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  22. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
     [Google Scholar]
  23. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  24. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  25. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  26. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
     [Google Scholar]
  27. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
     [Google Scholar]
  28. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
     [Google Scholar]
  29. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–218 [View Article]
     [Google Scholar]
  30. Bernardet J-F, Nakagawa Y. An introduction to the family Flavobacteriaceae. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E. et al. (editors) The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd ed. vol. 7 New York, NY: Springer; 2006 pp. 455–480
     [Google Scholar]
  31. Bernardet J-F. Family I. Flavobacteriaceae Reichenbach 1992. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ. et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 4 New York, NY: Springer; 2011 pp. 106–111
     [Google Scholar]
  32. Murray RGE, Doetsch RN, Robinow CF. Determinative and cytological light microscopy. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 21–41
     [Google Scholar]
  33. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  34. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  35. Zhang H, Cheng MG, Sun B, Guo SH, Song M et al. Flavobacterium suzhouense sp. nov., isolated from farmland river sludge. Int J Syst Evol Microbiol 2015; 65:370–374 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001804
Loading
Flavobacterium arcticum sp. nov., isolated from Arctic seawater
Int J Syst Evol Microbiol 67, 1070 (2017); https://doi.org/10.1099/ijsem.0.001804
/content/journal/ijsem/10.1099/ijsem.0.001804
/content/journal/ijsem/10.1099/ijsem.0.001804
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error