sp. nov., isolated from a household product plant Free

Abstract

A Gram-stain-negative, rod-shaped, bright-yellow-pigmented bacterium, designated 164, was isolated from a used sponge for equipment cleaning at a household product plant in China. The 16S rRNA gene sequence comparisons indicated that strain 164 was most closely related to DSM 22890 (98.28 % similarity) and shared sequence similarities of 97.73–98.27 % with other members of the genus . In DNA–DNA hybridization studies the relatedness between strain 164 and its closest phylogenetic neighbours was <70 %, which indicated that strain 164 represented a novel species of the genus . The DNA G+C content of strain 164 was 65.9 mol%. The major respiratory quinone was ubiquinone Q-10 (83.5 %) with minor amounts of Q-9 (16.5 %). The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine, unidentified aminolipids and unidentified aminophospholipids. Spermidine was the major polyamine. The major fatty acids were summed feature 8 (consisting of Cω7 and/or Cω6) and C 2-OH. The results obtained from phylogenetic analysis, DNA–DNA hybridization, and chemotaxonomic and phenotypic analysis support the conclusion that strain 164 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 164 (=CICC 11035s=DSM 103351).

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2017-09-01
2024-03-28
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