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Abstract

To better characterize murine intestinal microbiota, a large number (187) of Gram-positive-staining, rod- and coccoid-shaped, and facultatively or strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Group 1, as represented by strain NYU-BL-A3, was most closely related to , with 16S rRNA gene sequence similarity values of 87.7 %. A second group, represented by NYU-BL-A4, was most closely related to , with 86.6 % 16S rRNA gene sequence similarity. A third group had a nearly identical 16S rRNA gene sequence (99.9 %) compared with the recently described , also recovered from a laboratory mouse; however, this strain had a few differences in biochemical characteristics, which are detailed in an emended description. The predominant (>10 %) cellular fatty acids of strain NYU-BL-A3 were C and C, and those of strain NYU-BL-A4 were C, C, C and Cω9. The two groups could also be distinguished by multiple biochemical reactions, with the group represented by NYU-BL-A4 being considerably more active. Based on phylogenetic, biochemical and chemotaxonomic criteria, two novel genera are proposed, gen. nov., sp. nov. with NYU-BL-A3 (=ATCC TSD-63=DSM 103668) as the type strain and gen. nov., sp. nov. with NYU-BL-A4 (=ATCC TSD-64=DSM 103457) as the type strain.

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2017-05-01
2020-01-25
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