1887

Abstract

Two bacterial strains, designated EGH7 and TSAH33, were isolated from human faeces and characterized by using a polyphasic taxonomic approach that included analysis of morphology, phenotypic and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA and gene sequence analyses. The results of 16S rRNA gene sequence analysis indicated that these strains represented members of the family and formed a monophyletic cluster near JCM 6483 (95 % sequence similarity), JCM 6515 (95 %), JCM 1429 (95 %), JCM 31501 (95 %) and JCM 31500 (94 %). The results of a gene sequence analysis supported the phylogenetic tree based on the 16S rRNA gene sequence, with a sequence similarity value of between 77.9 and 84.8 % to the five strains listed above. The novel strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-positive cocco-bacilli. The strains formed characteristic umbilicated colonies on EG agar plates. The major cellular fatty acids were Cω9, C and Cω9 dimethyl acetal (DMA). EGH7 and TSAH33 have DNA G+C contents of 46.9 and 45.5 mol%, respectively. On the basis of these data, strains EGH7 and TSAH33 represent a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is EGH7 (=JCM 30896=DSM 103426).

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2017-05-01
2021-10-27
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