A Gram-stain-positive, non-motile, non-spore-forming, small spherical bacterium, strain S31T, was isolated from skin surface (external ear lobe) of a healthy human subject and characterized using a polyphasic approach. On the basis of 1507 bp 16S rRNA gene sequence comparison, S31T showed highest (92.8 %, AY119686) sequence similarity with Macrococcus brunensis CCUG 47200T followed by Macrococcus caseolyticus DSM 20597T (92.7 % AP009484) and formed a separate clade with 65 % bootstrap support. The DNA G+C content was found to be 34 mol%. Anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 are the predominant fatty acids in fatty acid methyl ester (FAME) profile of strain S31T. It contained A3α type peptidoglycan with l-Lys–Gly3–l-Ala peptide. Comparative study of morphological and physiological traits indicated that S31T has phenetically diverged from its closest relatives. On the basis of morphological, chemotaxonomic and genotypic data, S31T showed marked distinctions from its closest relatives of the family Staphylococcaceae and is proposed to represent a novel genus Auricoccus with Auricoccus indicus as type species of the genus. S31T (CCUG 69858T=KCTC 33611T=MCC 3027T) is the type strain of the species.
NaikS, BouladouxN, WilhelmC, MolloyMJ, SalcedoR et al. Compartmentalized control of skin immunity by resident commensals. Science2012; 337:1115–1119 [View Article][PubMed]
PrakashO, VermaM, SharmaP, KumarM, KumariK et al. Polyphasic approach of bacterial classification – an overview of recent advances. Indian J Microbiol2007; 47:98–108 [View Article][PubMed]
PrakashO, MunotH, NimonkarY, SharmaM, KumbhareS et al. Description of Pelistega indica sp. nov., isolated from human gut. Int J Syst Evol Microbiol2014; 64:1389–1394 [View Article][PubMed]
TindallBJ, Rosselló-MóraR, BusseHJ, LudwigW, KämpferP. Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol2010; 60:249–266 [View Article][PubMed]
GonzalezJM, Saiz-JimenezC. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol2002; 4:770–773[PubMed][CrossRef]
KloosWE, BallardDN, GeorgeCG, WebsterJA, HubnerRJ et al. Delimiting the genus Staphylococcus through description of Macrococcus caseolyticus gen. nov., comb. nov. and Macrococcus equipercicus sp. nov., and Macrococcus bovicus sp. nov. and Macrococcus carouselicus sp. nov. Int J Syst Bacteriol1998; 48:859–877 [View Article][PubMed]
AmoozegarMA, BagheriM, Makhdoumi-KakhkiA, DidariM, SchumannP et al.Aliicoccus persicus gen. nov., sp. nov., a halophilic member of the firmicutes isolated from a hypersaline lake. Int J Syst Evol Microbiol2014; 64:1964–1969 [View Article][PubMed]
YoonJH, LeeKC, WeissN, KangKH, ParkYH. Jeotgalicoccus halotolerans gen. nov., sp. nov. and Jeotgalicoccus psychrophilus sp. nov., isolated from the traditional Korean fermented seafood jeotgal. Int J Syst Evol Microbiol2003; 53:595–602 [View Article][PubMed]
AlvesM, NogueiraC, de Magalhães-Sant'anaA, ChungAP, MoraisPV et al.Nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline solution used in wound cleansing. Int J Syst Evol Microbiol2008; 58:2939–2944 [View Article][PubMed]