1887

Abstract

A Gram-stain-positive, non-motile, non-spore-forming, small spherical bacterium, strain S31, was isolated from skin surface (external ear lobe) of a healthy human subject and characterized using a polyphasic approach. On the basis of 1507 bp 16S rRNA gene sequence comparison, S31 showed highest (92.8 %, AY119686) sequence similarity with Macrococcus brunensis CCUG 47200 followed by Macrococcus caseolyticus DSM 20597 (92.7 % AP009484) and formed a separate clade with 65 % bootstrap support. The DNA G+C content was found to be 34 mol%. Anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 are the predominant fatty acids in fatty acid methyl ester (FAME) profile of strain S31. It contained A3α type peptidoglycan with l-Lys–Gly3 –l-Ala peptide. Comparative study of morphological and physiological traits indicated that S31 has phenetically diverged from its closest relatives. On the basis of morphological, chemotaxonomic and genotypic data, S31 showed marked distinctions from its closest relatives of the family Staphylococcaceae and is proposed to represent a novel genus Auricoccus with Auricoccus indicus as type species of the genus. S31 (CCUG 69858=KCTC 33611=MCC 3027) is the type strain of the species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001787
2017-05-30
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/5/1212.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001787&mimeType=html&fmt=ahah

References

  1. Grice EA, Segre JA. The skin microbiome. Nat Rev Microbiol 2011;9:244–253 [CrossRef][PubMed]
    [Google Scholar]
  2. Fredricks DN. Microbial ecology of human skin in health and disease. J Investig Dermatol Symp Proc 2001;6:167–169 [CrossRef][PubMed]
    [Google Scholar]
  3. Pflughoeft KJ, Versalovic J. Human microbiome in health and disease. Annu Rev Pathol 2012;7:99–122 [CrossRef][PubMed]
    [Google Scholar]
  4. Naik S, Bouladoux N, Wilhelm C, Molloy MJ, Salcedo R et al. Compartmentalized control of skin immunity by resident commensals. Science 2012;337:1115–1119 [CrossRef][PubMed]
    [Google Scholar]
  5. Mannerová S, Pantůcek R, Doskar J, Svec P, Snauwaert C et al. Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas. Int J Syst Evol Microbiol 2003;53:1647–1654 [CrossRef][PubMed]
    [Google Scholar]
  6. Prakash O, Verma M, Sharma P, Kumar M, Kumari K et al. Polyphasic approach of bacterial classification – an overview of recent advances. Indian J Microbiol 2007;47:98–108 [CrossRef][PubMed]
    [Google Scholar]
  7. Prakash O, Nimonkar Y, Shouche YS. Practice and prospects of microbial preservation. FEMS Microbiol Lett 2013;339:1–9 [CrossRef][PubMed]
    [Google Scholar]
  8. Prakash O, Munot H, Nimonkar Y, Sharma M, Kumbhare S et al. Description of Pelistega indica sp. nov., isolated from human gut. Int J Syst Evol Microbiol 2014;64:1389–1394 [CrossRef][PubMed]
    [Google Scholar]
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  10. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680[PubMed][CrossRef]
    [Google Scholar]
  11. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783[CrossRef]
    [Google Scholar]
  13. Tindall BJ, Rosselló-Móra R, Busse HJ, Ludwig W, Kämpfer P. Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 2010;60:249–266 [CrossRef][PubMed]
    [Google Scholar]
  14. Prakash O, Nimonkar Y, Munot H, Sharma A, Vemuluri VR et al. Description of Micrococcus aloeverae sp. nov., an endophytic actinobacterium isolated from Aloe vera. Int J Syst Evol Microbiol 2014;64:3427–3433 [CrossRef][PubMed]
    [Google Scholar]
  15. Smibert RM, Krieg NR. Phenotypic characterization. In Methods Gen Mol Bacteriol 1994; pp.607–654
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc; 2001
    [Google Scholar]
  17. Schumann P. 5 – Peptidoglycan structure. In Methods Microbiol 2011; pp.101–129
    [Google Scholar]
  18. Marmur J. A procedure for isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218[CrossRef]
    [Google Scholar]
  19. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002;4:770–773[PubMed][CrossRef]
    [Google Scholar]
  20. Kloos WE, Ballard DN, George CG, Webster JA, Hubner RJ et al. Delimiting the genus Staphylococcus through description of Macrococcus caseolyticus gen. nov., comb. nov. and Macrococcus equipercicus sp. nov., and Macrococcus bovicus sp. nov. and Macrococcus carouselicus sp. nov. Int J Syst Bacteriol 1998;48:859–877 [CrossRef][PubMed]
    [Google Scholar]
  21. Amoozegar MA, Bagheri M, Makhdoumi-Kakhki A, Didari M, Schumann P et al. Aliicoccus persicus gen. nov., sp. nov., a halophilic member of the firmicutes isolated from a hypersaline lake. Int J Syst Evol Microbiol 2014;64:1964–1969 [CrossRef][PubMed]
    [Google Scholar]
  22. Yoon JH, Lee KC, Weiss N, Kang KH, Park YH. Jeotgalicoccus halotolerans gen. nov., sp. nov. and Jeotgalicoccus psychrophilus sp. nov., isolated from the traditional Korean fermented seafood jeotgal. Int J Syst Evol Microbiol 2003;53:595–602 [CrossRef][PubMed]
    [Google Scholar]
  23. Ventosa A, Marquez MC, Ruiz-Berraquero F, Kocur M. Salinicoccus roseus gen. nov., sp. nov., a new moderately halophilic Gram-positive coccus. Syst Appl Microbiol 1990;13:29–33[CrossRef]
    [Google Scholar]
  24. Alves M, Nogueira C, de Magalhães-Sant'ana A, Chung AP, Morais PV et al. Nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline solution used in wound cleansing. Int J Syst Evol Microbiol 2008;58:2939–2944 [CrossRef][PubMed]
    [Google Scholar]
  25. Hoyles L, Collins MD, Foster G, Falsen E, Schumann P. Jeotgalicoccus pinnipedialis sp. nov., from a southern elephant seal (Mirounga leonina). Int J Syst Evol Microbiol 2004;54:745–748 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001787
Loading
/content/journal/ijsem/10.1099/ijsem.0.001787
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error