RT Journal Article SR Electronic(1) A1 Lai, Hangxian A1 Jiang, Yingying A1 Chen, Xiu A1 Li, Qinyuan A1 Jiang, Chenglin A1 Jiang, Yi A1 Wei, XiaominYR 2017 T1 Haloactinomyces albus gen. nov., sp. nov., isolated from the Dead Sea JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 5 SP 1163 OP 1168 DO https://doi.org/10.1099/ijsem.0.001783 PB Microbiology Society, SN 1466-5034, AB A novel halophilic, filamentous actinomycete strain, designated AFM 10258T, was isolated from a sediment sample collected from the Dead Sea of Israel. The isolate grew with 10–35 % NaCl and did not grow without NaCl. The isolate formed white aerial mycelium and long spore chains, and two spores were separated by sterile mycelium. The spores were non-motile, spherical and rugose-surfaced. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose as the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and three unknown phospholipids. Major fatty acids were anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0. MK-9(H4) was the predominant menaquinone and the DNA G+C content was 62.8 mol%. 16S rRNA gene sequence analysis indicated that strain AFM 10258T shared low sequence similarity with the closely related representatives of the families Pseudonocardiaceae (below 94.47 %) and Actinopolysporaceae (below 93.76 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AFM 10258T formed a robust clade with members of the family Actinopolysporaceae . On the basis of analysis of phenotypic, chemical and molecular characteristics, strain AFM 10258T is considered to represent a novel species of a new genus, for which the name Haloactinomyces albus gen. nov., sp. nov., is proposed. The type strain is AFM 10258T (=DSM 45977T=CGMCC 4.7115T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001783