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Abstract

Strain XMU 110, isolated from the rhizosphere soil of a flowering tree, Ceiba speciosa, was characterized by polyphasic taxonomy. Phylogenetic analysis based on 16S rRNA gene comparisons revealed that strain XMU 110 showed the highest similarity of 97.9 % to Nonomuraea jabiensis DSM 45507, and indicated the closest relatives were Nonomuraea roseoviolacea subsp. roseoviolacea ATCC 27297 (97.8 % 16S rRNA gene sequence similarity) and Nonomuraea salmonea DSM 43678 (97.4 %) after a neighbour-joining analysis. The phenotypic characteristics, as well as the DNA–DNA relatedness values between strain XMU 110 and N. roseoviolacea subsp . roseoviolacea ATCC 27297 (48.07±1.99 %) and N. salmonea DSM 43678 (40.55±8.30 %), distinguished the novel strain from its closest phylogenetic neighbours. The morphological, physiological and chemotaxonomic characteristics such as phospholipid type, diagnostic diamino acid of the peptidoglycan, whole-cell sugars, major menaquinones and major fatty acids further supported the assignment of strain XMU 110 to the genus Nonomuraea . The G+C content of the genomic DNA was 66.2 mol%. Based on the taxonomic data, strain XMU 110represents a novel species of the genus Nonomuraea , for which the name Nonomuraea ceibae sp. nov. is proposed. The type strain is XMU 110 (=MCCC 1K03213= KCTC 39826).

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2017-05-26
2019-10-18
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