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Abstract

Two closely related thermophilic bacterial strains, designated YIM 73013 and YIM 73008, were isolated from a sediment sample collected from a hot spring in Tibet, western Tibet province, China. The taxonomic positions of the two isolates were investigated using a polyphasic approach. The novel isolates were Gram-stain-negative, aerobic, short-rod-shaped and motile by means of a polar flagellum. They were oxidase- and catalase-positive and were able to grow at 30–55 °C (optimum, 37–45 °C), at pH 6.0–8.0 (optimum, pH 7.0) and with NaCl tolerance up to 1 % (w/v). Phylogenetic analyses based on 16S rRNA gene sequences showed that strains YIM 73013 and YIM 73008 formed a distinct lineage with respect to closely related genera in the family and shared highest 16S rRNA gene sequences similarities with R-24608 (96.3 and 96.4 %, respectively). The respiratory quinone was ubiquinone-8 (Q-8) and the major cellular fatty acids observed were Cω6, C and summed feature 3 (Cω7 and/or Cω). The genomic DNA G+C contents of strains YIM 73013 and YIM 73008 were 68.7 and 68.3 mol%, respectively. Based on the morphological, phylogenetic and chemotaxonomic results, the two isolates represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is YIM 73013 (=DSM 101684=KCTC 42873).

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2017-05-01
2020-01-27
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