1887

Abstract

A novel bacterial strain, MVW-6, was isolated from a freshwater spring in Taiwan and characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-6 belonged to the genus and showed the highest levels of sequence similarity to CL-GP79 (98.2 % 16S rRNA gene sequence similarity) and MIB-4 (97.4 %). Cells of strain MVW-6 were Gram-stain-negative, aerobic, non-motile rods that were covered by large capsules and formed light pink colonies. Growth occurred at 15–37 °C (optimum 15–25 °C), at pH 5–9 (optimum pH 7) and with 0–0.5 % (w/v) NaCl (optimum 0 %). Strain MVW-6 contained iso-C, summed feature 3 (Cω7 and/or Cω6), Cω5 and C as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, five unidentified aminophospholipids, one unidentified glycolipid, one unidentified phospholipid, one unidentified aminolipid and two unidentified lipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 39.5 mol%. DNA–DNA hybridization values for strain MVW-6 with CL-GP79 and MIB-4 were less than 50 %. On the basis of the phylogenetic inference and phenotypic data, strain MVW-6 a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MVW-6 (=BCRC 80975=LMG 29555=KCTC 33800).

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2017-05-01
2020-01-24
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