1887

Abstract

Two slightly yellowish-pigmented, oxidase-negative, rod-shaped and Gram-stain-negative bacterial strains (30TX1 and DL20), isolated from and , respectively, during soil sampling in Vietnam were studied using a polyphasic taxonomic approach. Strain 30TX1 showed highest 16S rRNA gene sequence similarity to the type strain of (98.9 %) and strain DL20 to that of (98.7 %). Sequence similarities to all other species were lower (<98.4 %). The two strains shared 98 % 16S rRNA gene sequence similarity. Multilocus sequence analysis (MLSA) based on concatenated partial , , , and gene sequences showed a clear distinction of strains 30TX1 and DL20 among each other and to the closest related type strains. DNA–DNA hybridizations between strain DL20 and the type strain of resulted in a relatedness value of 53 %. Genome-to-genome-based comparisons gave average nucleotide identities of 93.6 % (reciprocal 93.5 %) for strain 30TX1 and DSM 16337, of 92.8 % (reciprocal 93 %) for strain DL20 and DSM 22670and of 93.0 % (reciprocal 93.2 %) for the two novel strains. The fatty acid profile of the strains consisted of the major fatty acids C, C, C cyclo, Cω7 and/or iso-C 2-OH, and C 7 Genome-to-genome comparison and MLSA results together with the differential biochemical and chemotaxonomic properties showed that strains 30TX1 and DL20 represent novel species, for which the names sp. nov. (type strain 30TX1=CCM 8727=LMG 29916) and sp. nov. (type strain DL20=CCM 8728=LMG 29917) are proposed, respectively.

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2017-05-01
2020-01-28
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