1887

Abstract

A novel, extremely halophilic archaeon, strain Y73, was isolated from a salt mine in Yunnan, China. Colonies formed on solid medium were circular (2–3 mm in diameter), smooth, orange, glistening and convex (~1 mm in elevation). Cells were Gram-stain-negative, non-motile and pleomorphic. Mg was required for growth (optimum at 0.05 M). Optimal growth was observed at 20 % (w/v) NaCl, 42–45 °C and pH 7.5–8.5 under aerobic conditions. 16S rRNA gene sequence comparison showed that strain Y73 was closely related to B8 (similarity: 98.1 %), 9-3 (97.9 %) and JCM 8865 (97.6 %). Levels of gene sequence similarity between strain Y73 and B8, 9-3 and JCM 8865 were 93.6, 93.8 and 94.7 %, respectively. DNA–DNA relatedness between strain Y73 and B8 was 38.7±0.5 %, while that between strain Y73 and JCM 8865 was 31.0±0.9 % . The DNA G+C content of strain Y73 was 61.9 mol%. The major polar lipids of strain Y73 were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate as phospholipids, and sulfated diglycosyl diether-1 as glycolipid. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain Y73 represents a novel species, for which the name sp. nov is proposed. The type strain is Y73 (=CGMCC 1.15503=JCM 31271).

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2017-05-01
2024-10-03
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