1887

Abstract

A novel, extremely halophilic archaeon, strain Y73, was isolated from a salt mine in Yunnan, China. Colonies formed on solid medium were circular (2–3 mm in diameter), smooth, orange, glistening and convex (~1 mm in elevation). Cells were Gram-stain-negative, non-motile and pleomorphic. Mg was required for growth (optimum at 0.05 M). Optimal growth was observed at 20 % (w/v) NaCl, 42–45 °C and pH 7.5–8.5 under aerobic conditions. 16S rRNA gene sequence comparison showed that strain Y73 was closely related to Halorubrum halophilum B8 (similarity: 98.1 %), Halorubrum lipolyticum 9-3 (97.9 %) and Halorubrum saccharovorum JCM 8865 (97.6 %). Levels of rpoB′ gene sequence similarity between strain Y73 and H. halophilum B8, H. lipolyticum 9-3 and H. saccharovorum JCM 8865 were 93.6, 93.8 and 94.7 %, respectively. DNA–DNA relatedness between strain Y73 and H. halophilum B8 was 38.7±0.5 %, while that between strain Y73 and H. saccharovorum JCM 8865 was 31.0±0.9 % . The DNA G+C content of strain Y73 was 61.9 mol%. The major polar lipids of strain Y73 were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate as phospholipids, and sulfated diglycosyl diether-1 as glycolipid. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain Y73 represents a novel species, for which the name Halorubrum trueperi sp. nov is proposed. The type strain is Y73 (=CGMCC 1.15503=JCM 31271).

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2017-05-04
2019-10-15
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References

  1. Oren A. Halophilic microbial communities and their environments. Curr Opin Biotechnol 2015;33:119–124 [CrossRef][PubMed]
    [Google Scholar]
  2. Ventosa A, de La Haba RR, Sánchez-Porro C, Papke RT. Microbial diversity of hypersaline environments: a metagenomic approach. Curr Opin Microbiol 2015;25:80–87 [CrossRef][PubMed]
    [Google Scholar]
  3. Boetius A, Joye S. Ecology. Thriving in salt. Science 2009;324:1523–1525 [CrossRef][PubMed]
    [Google Scholar]
  4. Xiao W, Wang ZG, Wang YX, Schneegurt MA, Li ZY et al. Comparative molecular analysis of the prokaryotic diversity of two salt mine soils in southwest China. J Basic Microbiol 2013;53:942–952 [CrossRef][PubMed]
    [Google Scholar]
  5. Chen S, Liu HC, Zhao D, Yang J, Zhou J et al. Halorubrum yunnanense sp. nov., isolated from a subterranean salt mine. Int J Syst Evol Microbiol 2015;65:4526–4532 [CrossRef][PubMed]
    [Google Scholar]
  6. Chen S, Liu HC, Zhou J, Xiang H. Haloparvum sedimenti gen. nov., sp. nov., a member of the family Haloferacaceae. Int J Syst Evol Microbiol 2016a;66:2327–2334 [CrossRef][PubMed]
    [Google Scholar]
  7. Chen S, Liu HC, Zhou J, Xiang H. Halorubrum pallidum sp. nov., an extremely halophilic archaeon isolated from a subterranean rock salt. Int J Syst Evol Microbiol 2016b;66:2980–2986 [CrossRef][PubMed]
    [Google Scholar]
  8. Li Y, Xiang H, Liu J, Zhou M, Tan H. Purification and biological characterization of halocin C8, a novel peptide antibiotic from Halobacterium strain AS7092. Extremophiles 2003;7:401–407 [CrossRef][PubMed]
    [Google Scholar]
  9. Oren A, Ventosa A, Grant WD. Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 1997;47:233–238 [CrossRef]
    [Google Scholar]
  10. Dussault HP. An improved technique for staining red halophilic bacteria. J Bacteriol 1955;70:484–485[PubMed]
    [Google Scholar]
  11. Gonzalez C, Gutierrez C, Ramirez C. Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 1978;24:710–715 [CrossRef][PubMed]
    [Google Scholar]
  12. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. (editor) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  13. Cui HL, Lin ZY, Dong Y, Zhou PJ, Liu SJ. Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 2007;57:2204–2206 [CrossRef][PubMed]
    [Google Scholar]
  14. Mcgenity TJ, Grant WD. Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb. nov., Halorubrum sodomense comb. nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst Appl Microbiol 1995;18:237–243 [CrossRef]
    [Google Scholar]
  15. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  16. Cui HL, Zhou PJ, Oren A, Liu SJ. Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles 2009;13:31–37 [CrossRef][PubMed]
    [Google Scholar]
  17. Minegishi H, Kamekura M, Itoh T, Echigo A, Usami R et al. Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B' (rpoB') gene. Int J Syst Evol Microbiol 2010;60:2398–2408 [CrossRef][PubMed]
    [Google Scholar]
  18. Han D, Cui HL. Halorubrum laminariae sp. nov., isolated from the brine of salted brown alga Laminaria. Antonie van Leeuwenhoek 2015;107:217–223 [CrossRef][PubMed]
    [Google Scholar]
  19. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  20. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 1999;41:95–98
    [Google Scholar]
  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  22. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218 [CrossRef]
    [Google Scholar]
  23. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962;5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  24. Owen RJ, Hill LR. The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Skinner FA, Lovelock DW. (editors) Identification Methods for Microbiologists, 2nd ed. London: Academic Press; 1979; pp.217–296
    [Google Scholar]
  25. Grant WD, Kamekura M, McGenity TJ, Ventosa A. Order I. Halobacteriales Grant & Larsen 1989b, 495VP (Effective publication: Grant & Larsen 1989a, 2216). In Boone DR, Castenholz RW, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 1 New York: Springer; 2001; pp.294–334
    [Google Scholar]
  26. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970;12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  27. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  28. Yim KJ, Cha IT, Lee HW, Song HS, Kim KN et al. Halorubrum halophilum sp. nov., an extremely halophilic archaeon isolated from a salt-fermented seafood. Antonie van Leeuwenhoek 2014;105:603–612 [CrossRef][PubMed]
    [Google Scholar]
  29. Cui HL, Tohty D, Zhou PJ, Liu SJ. Halorubrum lipolyticum sp. nov. and Halorubrum aidingense sp. nov., isolated from two salt lakes in Xin-Jiang, China. Int J Syst Evol Microbiol 2006;56:1631–1634 [CrossRef][PubMed]
    [Google Scholar]
  30. Tomlinson GA, Hochstein LI. Halobacterium saccharovorum sp. nov., a carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 1976;22:587–591 [CrossRef][PubMed]
    [Google Scholar]
  31. Gutiérrez MC, Castillo AM, Pagaling E, Heaphy S, Kamekura M et al. Halorubrum kocurii sp. nov., an archaeon isolated from a saline lake. Int J Syst Evol Microbiol 2008;58:2031–2035 [CrossRef][PubMed]
    [Google Scholar]
  32. Franzmann PD, Stackebrandt E, Sanderson K, Volkman JK, Cameron DE et al. Halobacterium lacusprofundi sp. nov., a halophilic bacterium isolated from Deep Lake, Antarctica. Syst Appl Microbiol 1988;11:20–27 [CrossRef]
    [Google Scholar]
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