1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 5

gen. nov., sp. nov. a novel planctomycete associated with macroalgae Free

Abstract

One strain of a novel genus and species of the order , designated FC18, was isolated from the epiphytic community of . This strain was non-pigmented in medium M13 but was slightly pink pigmented on medium M14, containing four-fold the levels of glucose, peptone and yeast extract of M13. The organism was primarily spherical, with unicellular non-motile forms and rosettes. The optimal temperature for growth was about 25 °C and the optimal pH was 7.5. FC18 was chemoorganotrophic and aerobic. Several sugars, polyols and amino acids were assimilated. The major fatty acids were Cω9, C and C. The major polar lipids were phosphatidylglycerol (PG) and two unknown lipids. Menaquinone 5 (MK-5) was the main respiratory quinone, but MK-6 was also present. The results of the 16S rRNA gene sequence analysis confirmed the affiliation of this organism to the order family , with as the closest relative with only 86 % sequence similarity. On the basis of physiological, biochemical and chemotaxonomic characteristics we propose that FC18(=LMG 29748=DSM 26290) represents a novel species of a novel genus of the family for which we propose the name gen. nov., sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): macroalgae , menaquinone 5 , new genus and species and Planctomycetes
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001760
2017-05-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/5/1571.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001760&mimeType=html&fmt=ahah

References

  1. Hollants J, Leliaert F, de Clerck O, Willems A. What we can learn from sushi: a review on seaweed–bacterial associations. FEMS Microbiol Ecol 2013; 83:1–16 [View Article][PubMed]
     [Google Scholar]
  2. Lage OM, Bondoso J. Planctomycetes and macroalgae, a striking association. Front Microbiol 2014; 5:267 [View Article][PubMed]
     [Google Scholar]
  3. Bondoso J, Balagué V, Gasol JM, Lage OM. Community composition of the Planctomycetes associated with different macroalgae. FEMS Microbiol Ecol 2014; 88:445–456 [View Article][PubMed]
     [Google Scholar]
  4. Bengtsson MM, Øvreås L. Planctomycetes dominate biofilms on surfaces of the kelp Laminaria hyperborea. BMC Microbiol 2010; 10:261 [View Article][PubMed]
     [Google Scholar]
  5. Lage OM, Bondoso J. Planctomycetes diversity associated with macroalgae. FEMS Microbiol Ecol 2011; 78:366–375 [View Article][PubMed]
     [Google Scholar]
  6. Wegner CE, Richter-Heitmann T, Klindworth A, Klockow C, Richter M et al. Expression of sulfatases in Rhodopirellula baltica and the diversity of sulfatases in the genus Rhodopirellula. Mar Genomics 2013; 9:51–61 [View Article][PubMed]
     [Google Scholar]
  7. Lachnit T, Meske D, Wahl M, Harder T, Schmitz R. Epibacterial community patterns on marine macroalgae are host-specific but temporally variable. Environ Microbiol 2011; 13:655–665 [View Article][PubMed]
     [Google Scholar]
  8. Lyman J, Fleming R. Composition of artificial seawater. J Mar Res 1940; 450:134–146
     [Google Scholar]
  9. Schlesner H, Rensmann C, Tindall BJ, Gade D, Rabus R et al. Taxonomic heterogeneity within the Planctomycetales as derived by DNA–DNA hybridization, description of Rhodopirellula baltica gen. nov., sp. nov., transfer of Pirellula marina to the genus Blastopirellula gen. nov. as Blastopirellula marina comb. nov. and emended description of the genus Pirellula. Int J Syst Evol Microbiol 2004; 54:1567–1580 [View Article][PubMed]
     [Google Scholar]
  10. Macdonell MT, Singleton FL, Hood MA. Diluent composition for use of API 20E in characterizing marine and estuarine bacteria. Appl Environ Microbiol 1982; 44:423–427[PubMed]
     [Google Scholar]
  11. Skerman VBD. Abstracts of Microbiological Methods New York: John Wiley & Sons; 1969
     [Google Scholar]
  12. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM. et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: American Society for Microbiology; 2007 pp. 330–393
     [Google Scholar]
  13. da Costa MS, Albuquerque L, Nobre MF, Wait R. The identification of polar lipids in prokaryotes. In Rainey FA, Oren A. (editors) Methods in Microbiology (Taxonomy of Prokaryotes) vol. 38 London, UK: Elsevier Ltd; 2011 pp. 165–181
     [Google Scholar]
  14. da Costa MS, Albuquerque L, Nobre MF, Wait R. The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. In Rainey FA, Oren A. (editors) Methods in Microbiology (Taxonomy of Prokaryotes) vol. 38 London, UK: Elsevier Ltd; 2011 pp. 197–206
     [Google Scholar]
  15. da Costa MS, Albuquerque L, Nobre MF, Wait R. The identification of fatty acids in bacteria. In Rainey FA, Oren A. (editors) Methods in Microbiology (Taxonomy of Prokaryotes) vol.38 London, UK: Elsevier Ltd; 2011 pp. 183–196
     [Google Scholar]
  16. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
     [Google Scholar]
  17. Nielsen P, Fritze D, Priest FG. Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 1995; 141:1745–1761 [View Article]
     [Google Scholar]
  18. Bondoso J, Goday-Vitorino F, Balagué V, Gasol JM, Harder J et al. Epiphytic Planctomycetes communities associated with three main lineages of macroalgae. FEMS Microbial Ecology 2017; 93:fiw255 [CrossRef]
     [Google Scholar]
  19. Schlesner H. Pirella marina sp. nov., a budding, peptidoglycan-less bacterium from brackish water. Syst Appl Microbiol 1986; 8:177–180 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001760
Loading
Mariniblastus fucicola gen. nov., sp. nov. a novel planctomycete associated with macroalgae
Int J Syst Evol Microbiol 67, 1571 (2017); https://doi.org/10.1099/ijsem.0.001760
/content/journal/ijsem/10.1099/ijsem.0.001760
/content/journal/ijsem/10.1099/ijsem.0.001760
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error