%0 Journal Article %A Xie, Fuhong %A Yang, Yang %A Ma, Huan %A Quan, Shujing %A Yue, Dandan %A Sun, Yufei %A Feng, Fei %A Zhen, Jing %A Liu, Dehai %T Nocardioides phosphati sp. nov., an actinomycete isolated from a phosphate mine %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 5 %P 1522-1528 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001754 %K phosphate mine %K actinobacteria %K nocardioides phosphati %I Microbiology Society, %X A Gram-staining-positive, non-spore-forming and non-motile strain, designated WYH11-7T, was isolated from a phosphate mine in Yunnan Province, PR China. The taxonomic position of WYH11-7T was investigated by polyphasic approaches. Phylogenetic analyses based on 16S rRNA gene sequences indicated that WYH11-7T represents a member of the genus Nocardioides . WYH11-7T was closely related to Nocardioidesjensenii DSM 20641T, Nocardioidesdubius DSM 19084T and Marmoricolaterrae DSM 27141T, and had pairwise 16S rRNA gene sequence similarities of 97.4, 97.2 and 97.0 %, respectively. DNA–DNA relatedness values between WYH11-7T and related type strains N. jensenii DSM 20641T and N. dubius DSM 19084T were found to be 17.6±4.9 and 14.6±3.1 %, respectively. The respiratory menaquinone of WYH11-7T was MK-8 (H4) while the major fatty acids were C18 : 1ω9c, C16 : 0, C17 : 0, C17 : 1ω8c, C18 : 1 10-methyl and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids. Whole-cell hydrolysates contained mannose, ribose, glucose and galactose along with ll-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 71.2 mol%. Phenotypic, phylogenetic and chemotaxonomic data indicated that strain WYH11-7T represents a novel species of the genus Nocardioides , for which the name Nocardioidesphosphatisp. nov. is proposed. The type strain is WYH11-7T (=CGMCC 4.7371T=DSM 104026T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001754