1887

Abstract

An actinobacterium, designated strain 9583b, was isolated from the lichen collected from Jiaozi Snow Mountain, Yunnan Province, China. Cells of strain 9583b were Gram-stain-positive, aerobic, catalase-positive and oxidase-negative. The strain have a short rod-shaped, irregular morphology, and could grow at the temperature range of 4 to 28 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 9583b belonged to the genus in the family , and shared highest sequence similarity with the type strains of and (96.8 and 95.6 %, respectively). The peptidoglycan type was B2γ, with diaminobutyric acid as the diagnostic diamino acid. The polar lipids comprised of phosphatidylglycerol, diphosphatidylglycerol, five unidentified glycolipids and three unidentified phospholipids. The respiratory quinone was determined to be MK-10. While the major fatty acids (>5 %) of strain 9583b were anteiso-C, C 2-OH and iso-C, the 1,1-dimethoxy-alkanes included a-15 : 0 DMA, i-16 : 0 DMA, a-17 : 0 DMA and i-15 : 0 DMA. The genomic DNA G+C content of strain 9583b was 66.8 mol%. On the basis of the phylogenetic, phenotypic and chemotaxonomic data in this study, strain 9583b represents a novel species of the genus S, for which the name sp. nov. is proposed. The type strain is 9583b (=CGMCC 1.12976=DSM 103962).

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2017-05-01
2020-01-26
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References

  1. Männistö MK, Schumann P, Rainey FA, Kämpfer P, Tsitko I et al. Subtercola boreus gen. nov., sp. nov. and Subtercola frigoramans sp. nov., two new psychrophilic actinobacteria isolated from boreal groundwater. Int J Syst Evol Microbiol 2000;50:1731–1739 [CrossRef][PubMed]
    [Google Scholar]
  2. Greene AC, Euzéby JP, Tindall BJ, Patel BK, Petal BKC. Proposal of Frondihabitans gen. nov. to replace the illegitimate genus name Frondicola Zhang, et al. 2007. Int J Syst Evol Microbiol 2009;59:447–448 [CrossRef][PubMed]
    [Google Scholar]
  3. Zhang L, Xu Z, Patel BK. Frondicola australicus gen. nov., sp. nov., isolated from decaying leaf litter from a pine forest. Int J Syst Evol Microbiol 2007;57:1177–1182 [CrossRef][PubMed]
    [Google Scholar]
  4. Cardinale M, Grube M, Berg G. Frondihabitans cladoniiphilus sp. nov., an actinobacterium of the family Microbacteriaceae isolated from lichen, and emended description of the genus Frondihabitans. Int J Syst Evol Microbiol 2011;61:3033–3038 [CrossRef][PubMed]
    [Google Scholar]
  5. Lee SD. Frondihabitans peucedani sp. nov., an actinobacterium isolated from rhizosphere soil, and emended description of the genus Frondihabitans Greene et al. 2009. Int J Syst Evol Microbiol 2010;60:1740–1744 [CrossRef][PubMed]
    [Google Scholar]
  6. Kim SJ, Lim JM, Ahn JH, Weon HY, Hamada M et al. Description of Galbitalea soli gen. nov., sp. nov., and Frondihabitans sucicola sp. nov. Int J Syst Evol Microbiol 2014;64:572–578 [CrossRef][PubMed]
    [Google Scholar]
  7. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340 [CrossRef]
    [Google Scholar]
  8. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp.115–175
    [Google Scholar]
  9. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  10. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  11. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  12. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  13. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  14. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  15. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  16. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988;38:358–361 [CrossRef]
    [Google Scholar]
  17. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207[CrossRef]
    [Google Scholar]
  18. Schumann P. Peptidoglycan structure. Methods Microbiol 2011;38:101–129[CrossRef]
    [Google Scholar]
  19. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218 [CrossRef]
    [Google Scholar]
  20. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962;5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  21. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972;36:407–477[PubMed]
    [Google Scholar]
  22. Murray RGE, Doetsch RN, Robinow CF. Determinative and cytological light microscopy. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.21–41
    [Google Scholar]
  23. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  24. Behrendt U, Ulrich A, Schumann P, Naumann D, Suzuki K. Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov. Int J Syst Evol Microbiol 2002;52:1441–1454 [CrossRef][PubMed]
    [Google Scholar]
  25. Evtushenko LI, Takeuchi M. The family Microbacteriaceae. In Dworkin SM, Falkow ER, Schleifer KH, Stackebrandt E. (editors) The Prokaryotes: A Handbook on the Biology of Bacteria New York, NY: Springer; 2006; pp.1020–1098
    [Google Scholar]
  26. Schumann P, Behrendt U, Ulrich A, Suzuki K. Reclassification of Subtercola pratensis Behrendt et al. 2002 as Agreia pratensis comb. nov. Int J Syst Evol Microbiol 2003;53:2041–2044 [CrossRef][PubMed]
    [Google Scholar]
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