sp. nov., isolated from a tidal flat Free

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-31, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-31 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-31 belonged to the genus , clustering with the type strains of and , sharing 98.1 % sequence similarity with each. Strain OITF-31 exhibited 16S rRNA gene sequence similarities of 93.9–95.8 % to the type strains of other species and less than of 91.8 % to the type strains of other recognized species. Strain OITF-31 contained MK-6 as the predominant menaquinone and iso-C 3-OH and iso-C as the major fatty acids. The major polar lipids detected in strain OITF-31 were phosphatidylethanolamine, six unidentified lipids and two unidentified aminolipids. The DNA G+C content of strain OITF-31 was 38.1 mol% and its mean DNA–DNA relatedness values with the type strains of and were 24 and 19 %, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-31 is separated from recognized species of the genus . On the basis of the data presented, strain OITF-31 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OITF-31 (=KCTC 52502=NBRC 112459).

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2017-05-01
2024-03-29
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