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Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-31, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-31 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-31 belonged to the genus Nonlabens , clustering with the type strains of Nonlabens dokdonensis and Nonlabens xylanidelens , sharing 98.1 % sequence similarity with each. Strain OITF-31 exhibited 16S rRNA gene sequence similarities of 93.9–95.8 % to the type strains of other Nonlabens species and less than of 91.8 % to the type strains of other recognized species. Strain OITF-31 contained MK-6 as the predominant menaquinone and iso-C17 : 0 3-OH and iso-C15 : 0 as the major fatty acids. The major polar lipids detected in strain OITF-31 were phosphatidylethanolamine, six unidentified lipids and two unidentified aminolipids. The DNA G+C content of strain OITF-31 was 38.1 mol% and its mean DNA–DNA relatedness values with the type strains of N. dokdonensis and N. xylanidelens were 24 and 19 %, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-31 is separated from recognized species of the genus Nonlabens . On the basis of the data presented, strain OITF-31 is considered to represent a novel species of the genus Nonlabens , for which the name Nonlabens aestuariivivens sp. nov. is proposed. The type strain is OITF-31 (=KCTC 52502=NBRC 112459).

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2017-05-25
2019-10-15
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