1887

Abstract

A straw-coloured, Gram-staining-negative, aerobic, motile and rod-shaped bacterium, designated strain K-1-15, was isolated from reclaimed grassland soil from Biratnagar, Morang, Nepal. This strain was non-spore-forming, catalase-negative and oxidase-positive. It was able to grow at 10–45 °C, pH 6.5–9.5 and 0–1.5 % (w/v) NaCl concentration. This strain was taxonomically characterized by a polyphasic approach. Based on the results of 16S rRNA gene sequence analysis, K-1-15 formed a distinct lineage within the family and was most closely related to members of the genera (96.99–95.34 % sequence similarity), (96.72–95.45 % sequence similarity) and (95.85 % sequence similarity). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major fatty acids of K-1-15 were summed feature 3 (Cω7 and/or Cω6), C summed feature 8 (Cω7 and/or Cω6), C 3-OH, and iso-C. The genomic DNA G+C content of this novel strain was 65.2 mol %. The DNA–DNA relatedness between K-1-15 and DSM 25712 and LMG 26149 were 18.3 and 13.7 % repectively. On the basis of the results of morphological, physiological, chemotaxonomic and phylogenetic analyses, K-1-15 represents a novel species of the genus in the family for which the name sp. nov. is proposed. The type strain is K-1-15 (=KEMB 9005-422=KACC 18909=JCM 31463). Based on new data obtained in this study, we also propose the reclassification of as comb. nov. (type strain JC206=CSUR P159=DSM 25712).

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2017-05-01
2021-08-01
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