1887

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Volume 67, Issue 5

gen. nov., sp. nov., a novel representative of the phylum Free

Abstract

A moderately thermophilic, anaerobic bacterium designated as strain KR was isolated from a shallow-water submarine hydrothermal vent (Kunashir Island, Southern Kurils, Russia). Cells of strain KR were thin (0.2–0.3 µm), flexible, motile, Gram-stain-negative rods of variable length. Optimal growth conditions were pH 6.6, 55 °C and 1–3 % (w/v) NaCl. Strain KR was able to ferment a wide range of proteinaceous substrates, pyruvate, and mono-, di- and polysaccharides. The best growth occurred with proteinaceous compounds. Nitrate significantly stimulated the growth on proteinaceous substrates decreasing H formation, ammonium being the main product of nitrate reduction. Strain KR did not need the presence of a reducing agent in the medium and tolerated the presence of oxygen in the gas phase up to 3 % (v/v). In the presence of nitrate, aerotolerance of isolate KR was enhanced up to 6–8 % O (v/v). Strain KR was able to grow chemolithoheterotrophically, oxidizing H and reducing nitrate to ammonium. Yeast extract (0.05 g l) was required for growth. The G+C content of the genomic DNA of strain KR was 47.3 mol%. 16S rRNA gene sequence analysis placed isolate KR in the phylum where it represented a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is KR (=DSM 101605=VKM B-3022).

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Keyword(s): anaerobic aerotolerant bacteria , Caldithrix , Calditrichaeota , Calorithrix , submarine hydrothermal vent and thermophily
© 2017 IUMS
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2017-05-01
2024-04-18
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Calorithrix insularis gen. nov., sp. nov., a novel representative of the phylum Calditrichaeota
Int J Syst Evol Microbiol 67, 1486 (2017); https://doi.org/10.1099/ijsem.0.001744
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