1887

Abstract

A bacterial strain designated RA9 was isolated from a root of Cistus ladanifer in Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate into the genus Bacillus with its closest relatives being Bacillus fortis R-6514 and Bacillus fordii R-7190 with 98.2 % similarity in both cases. DNA–DNA hybridization studies showed mean relatedness values of 29 and 30 %, respectively, between strain RA9 and the type strains of B. fortis and B. fordii . Cells of the isolate were Gram-stain-positive, motile, sporulating rods. Catalase and oxidase were positive. Gelatin, starch and casein were not hydrolysed. Menaquinone MK-7 was the only menaquinone detected and iso-C15 : 0 and anteiso-C15 : 0 were the major fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, one unidentifed glycolipid and one unidentified lipid. meso-Diaminopimelic acid was detected in the peptidoglycan. The DNA G+C content was 43.1 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain RA9 should be considered as representing a novel species of the genus Bacillus , for which the name Bacillus terrae sp. nov. is proposed. The type strain is RA9 (=LMG 29736=CECT 9170).

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2017-05-15
2019-12-06
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