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Volume 67, Issue 4

sp. nov., isolated from Pacific oyster Free

Abstract

A yellow, rod-shaped, Gram-reaction-negative bacterial strain, designated LPB0076, was isolated from a Pacific oyster. Results of 16S rRNA gene sequence analyses indicated that the strain represented a member of the genus . It had the highest sequence similarity to the type strains of (97.6 %) and (97.0 %), and its similarities with all other species of the genus were below 97.0 %. Its genome size (3.02 Mb), DNA G+C content (36.0 mol%), predominant cellular fatty acids (anteiso-C, iso-C and Cω7 and/or Cω6), and major polar lipid (phosphatidylethanolamine) were similar to those described previously for members of the genus . In contrast, a number of phenotypic characteristics, including the inability to grow microaerophilically, absence of flexirubin-type pigments and gliding motility and differences in enzymatic reactions, clearly distinguished LPB0076 from other species of the genus . The polyphasic data presented in this study indicate that this isolate should be classified as representing a novel species of the genus . The name sp. nov. is therefore proposed for the isolate, with the type strain being LPB0076 (=KACC 18706=JCM 31219).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteriodetes , Flavobacterium and Flavobacterium crassostreae
© 2017 IUMS
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2017-04-01
2024-04-19
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References

  1. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FM et al. (editors) Genus II. Flavobacterium gen. nov. In Bergey's Manual of Determinative Bacteriology, 1st ed. Baltimore, MD: Williams and Wilkins; 1923 pp. 97–117
     [Google Scholar]
  2. Bernardet JF, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Evol Microbiol 1996; 46:128–148 [View Article]
     [Google Scholar]
  3. Bernardet JF, Bowman JP. Genus I. Flavobacterium. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ. et al. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 4 New York: Springer; 2010 pp. 112–154
     [Google Scholar]
  4. Shin SK, Kim E, Choi S, Yi H. Cochleicola gelatinilyticus gen. nov., sp. nov., isolated from a marine gastropod, Reichia luteostoma. J Microbiol Biotechnol 2016; 26:1439–1445 [View Article][PubMed]
     [Google Scholar]
  5. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  6. Jeon YS, Lee K, Park SC, Kim BS, Cho YJ et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014; 64:689–691 [View Article][PubMed]
     [Google Scholar]
  7. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  8. Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003; 19:1572–1574 [View Article][PubMed]
     [Google Scholar]
  9. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA–DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
     [Google Scholar]
  10. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
     [Google Scholar]
  11. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  12. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Kreig NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  13. Shin SK, Goo H, Cho YJ, Kwon S, Yong D et al. Non-contiguous finished genome sequence and description of the gliding bacterium Flavobacterium seoulense sp. nov. Stand Genomic Sci 2014; 9:34 [View Article][PubMed]
     [Google Scholar]
  14. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
     [Google Scholar]
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Flavobacterium crassostreae sp. nov., isolated from Pacific oyster
Int J Syst Evol Microbiol 67, 988 (2017); https://doi.org/10.1099/ijsem.0.001728
/content/journal/ijsem/10.1099/ijsem.0.001728
/content/journal/ijsem/10.1099/ijsem.0.001728
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