1887

Abstract

A wine-red-coloured, Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain G-3-5, was isolated from oil-contaminated soil of Biratnagar, Morang, Nepal, during a study of oil-utilizing bacteria. This strain was catalase-negative and oxidase-positive. It was able to grow at 10–37 °C, at pH 6.0–10.0 and with 0.02–1.02 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain G-3-5 belongs to the genus and is closely related to CPCC 100056 (97.81 % sequence similarity), 5420S-30 (96.68 %), N75 (96.15 %), ‘’ R-1 (95.75 %) and 173/96 (95.30 %). The only respiratory quinone was ubiquinone-10. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major fatty acids of strain G-3-5 were summed feature 8 (Cω7 and/or Cω6), summed feature 3 (Cω7 and/or Cω6), C, C and C 2-OH. The genomic DNA G+C content of this novel strain was 68.3 mol%. The DNA–DNA relatedness between strain G-3-5 and KACC 13934 was 26.3 %. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain G-3-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is G-3-5 (=KEMB 9005-416=KACC 18908=JCM 31470).

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2017-04-01
2022-01-24
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