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Abstract

A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15. The organism was isolated from a soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum-quenching enrichment medium. Growth occurred at 4–30 °C, pH 6–11 and at moderately high salinity (0–15 %, w/v, NaCl), with optimal growth at 26 °C, at pH 7–8 and with 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain L10.15 belonged to the genus and was closely related to Or1 (99.3 % similarity), JH1 (99.0 %), DSM 14505 (98.3 %), AS/ASP6 (II) (97.6 %), TF-9 (97.5 %), ISL-6 (97.5 %) and NCIMB 629 (97.5 %). However, the average nucleotide identity-MUMmer analysis showed low genomic relatedness values of 71.1–81.7 % to the type strains of these closely related species of the genus . The principal fatty acids were anteiso-C : , Cω7 and anteiso-C, and the major menaquinones of strain L10.15 were MK-5 (48 %), MK-6 (6 %) and MK-7 (44 %). Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 39.4 mol%. The phenotypic and genotypic data indicate that strain L10.15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is L10.15 (=DSM 101994=KACC 18918).

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2017-04-01
2020-01-21
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