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Abstract

A novel Gram-stain-negative, rod-shaped, non-motile strain, designated GKX, was isolated from deep seawater. Strain GKX was able to grow at 20–35 °C (optimum, 25 °C), pH 5.5–9.5 (optimum, 7.5) and 0–4.0 % (w/v) NaCl (optimum, 1.0 %). The major fatty acids were Cω9 (15.4 %), C (18.4 %), C (12.0 %), iso-C (30.1 %) and anteiso-C (5.7 %). Strain GKX contained phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid as the main polar lipids. The only isoprenoid quinone was menaquinone-9. The diagnostic amino acids of the cell-wall peptidoglycan contained -diaminopimelic acid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GKX belonged to the genus in the family . The 16S rRNA gene sequence of this strain showed 98.0, 93.5 and 93.3 % sequence similarity, respectively, with those of MC 3726, A4T-83 and E100. DNA–DNA hybridization value of strain GKX with MC 3726 was 33.1 %. The G+C content of the genomic DNA was 59.5 mol%. On the basis of the genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain GKX was proposed to represent a novel species of the genus , named sp. nov. (type strain GKX=MCCC 1K03193=KCTC 52361).

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2017-03-01
2020-07-13
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