1887

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, non-flagellated, non-gliding, oxidase- and catalase-positive bacterium, designated CY01, was isolated from seawater of the Yellow Sea. CY01 grew at 15–37 °C (optimum, 30 °C), pH 5–8 (optimum, 6.5–7.5) and with 0.5–12 % (w/v) NaCl (optimum, 0.5–3.5 %). It could not produce flexirubin-type pigment or reduce nitrate to nitrite. CY01 showed the highest 16S rRNA gene sequence similarity to the type strain of (97.0 %) and clustered tightly with the species of the genus in the phylogenetic trees based on the 16S rRNA gene sequences. The major cellular fatty acids of CY01 were iso-C, iso-CG and iso-C 3-OH and the major respiratory quinone was menaquinone MK-6. Polar lipids included phosphatidylethanolamine (PE), four unidentified lipids and one unidentified aminolipid. The genomic DNA G+C content was 38.2 mol%. Based on the results of the polyphasic characterization of CY01, it represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CY01 (=CCTCC AB 2014348=KCTC 42440).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001712
2017-04-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/4/920.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001712&mimeType=html&fmt=ahah

References

  1. Lucena T, Pascual J, Giordano A, Gambacorta A, Garay E et al. Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae. Int J Syst Evol Microbiol 2010;60:2871–2876 [CrossRef][PubMed]
    [Google Scholar]
  2. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology [M] Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  3. Zhang XY, Zhang YJ, Yu Y, Li HJ, Gao ZM et al. Neptunomonas antarctica sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2010;60:1958–1961 [CrossRef][PubMed]
    [Google Scholar]
  4. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  5. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  6. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  7. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  8. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  10. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York: Academic Press; 1969; pp.21–132[CrossRef]
    [Google Scholar]
  11. Lin CY, Zhang XY, Liu A, Liu C, Song XY et al. Marivirga atlantica sp. nov., isolated from seawater and emended description of the genus Marivirga. Int J Syst Evol Microbiol 2015;65:1515–1519 [CrossRef][PubMed]
    [Google Scholar]
  12. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  13. Murray RGE, Doetsch RN, Robinow F. Determinative and cytological light microscopy. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp21–41
    [Google Scholar]
  14. Buck JD. Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 1982;44:992–993[PubMed]
    [Google Scholar]
  15. Bernardet JF, Nakagawa Y, Holmes B.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  16. Bernardet JF. Family I. Flavobacteriaceae Reichenbach 1992. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ. et al (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 4 New York: Springer; 2011; pp.106–111
    [Google Scholar]
  17. McBride MJ. The family Flavobacteriaceae. In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F. et al (editors) The Prokaryotes-Other Major Lineages of Bacteria and the Archaea, 4th ed.vol. 11 Berlin: Springer; 2014; pp.643–676
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001712
Loading
/content/journal/ijsem/10.1099/ijsem.0.001712
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error