1887

Abstract

Two novel anaerobic, mesophilic, biohydrogen-producing bacteria, designated strains ZGM211 and G1, were isolated from lake sediment. 16S rRNA and ATP synthase beta subunit (atp D) gene sequences and phylogenetic analysis of strains ZGM211 and G1 revealed an affiliation to the genus Clostridium sensu stricto (cluster I of the clostridia), with Clostridium acetobutylicum as the closest characterized species, showing the same sequence similarity of 96.4 % to the type strain (98.9 % between the two isolates). Cells of the two strains were rod shaped. Growth occurred at 20–45 °C, pH 4.0–8.0 and NaCl concentrations up to 2 % (w/v). Grown on glucose, the main fermentation products were H2, CO2, acetate and butyrate. The major fatty acids were C14 : 0 and C16 : 0. The DNA G+C contents of strains ZGM211 and G1 were 40.7 and 41.5 mol%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic differences, strains ZGM211 (=CICC 24070=BCRC 80950) and G1 (=CICC 24069=BCRC 80949) are proposed as the type strains of novel species of the genus Clostridium with the names Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001702
2017-04-03
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/3/710.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001702&mimeType=html&fmt=ahah

References

  1. Wiegel J, Tanner RS, Rainey FA. An Introduction to the Family Clostridiaceae New York, NY: Springer; 2006; pp.654–678
    [Google Scholar]
  2. Rainey FA, Holley BJ, Small A. Genus I. Clostridium Prazmowski 1880. In De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W et al. (editors) Bergey's Manual of Systematic Bacteriology. Volume 3: The Firmicutes USA: Springer; 2009; pp.738–828
    [Google Scholar]
  3. Wu YF, Zheng H, Wu QL, Yang H, Liu SJ. Clostridium algifaecis sp. nov., an anaerobic bacterial species from decomposing algal scum. Int J Syst Evol Microbiol 2014;64:3844–3848 [CrossRef][PubMed]
    [Google Scholar]
  4. Collins MD, Lawson PA, Willems A, Cordoba JJ, Fernandez-Garayzabal J et al. The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 1994;44:812–826 [CrossRef][PubMed]
    [Google Scholar]
  5. Lawson PA, Rainey FA. Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related species. Int J Syst Evol Microbiol 2016;66:1009–1016 [CrossRef]
    [Google Scholar]
  6. Song L, Dong X. Clostridium amylolyticum sp. nov., isolated from H2-producing UASB granules. Int J Syst Evol Microbiol 2008;58:2132–2135 [CrossRef][PubMed]
    [Google Scholar]
  7. Bowman KS, Dupré RE, Rainey FA, Moe WM. Clostridium hydrogeniformans sp. nov. and Clostridium cavendishii sp. nov., hydrogen-producing bacteria from chlorinated solvent-contaminated groundwater. Int J Syst Evol Microbiol 2010;60:358–363 [CrossRef][PubMed]
    [Google Scholar]
  8. Zhao X, Xing D, Fu N, Liu B, Ren N. Hydrogen production by the newly isolated Clostridium beijerinckii RZF-1108. Bioresour Technol 2011;102:8432–8436 [CrossRef][PubMed]
    [Google Scholar]
  9. Hahnke S, Striesow J, Elvert M, Mollar XP, Klocke M. Clostridium bornimense sp. nov., isolated from a mesophilic, two-phase, laboratory-scale biogas reactor. Int J Syst Evol Microbiol 2014;64:2792–2797 [CrossRef][PubMed]
    [Google Scholar]
  10. Hungate RE. A roll tube method for cultivation of strict anaerobes. Methods Microbiol 1969;3:117–132[CrossRef]
    [Google Scholar]
  11. Xing D, Ren N, Li Q, Lin M, Wang A et al. Ethanoligenens harbinense gen. nov., sp. nov., isolated from molasses wastewater. Int J Syst Evol Microbiol 2006;56:755–760 [CrossRef][PubMed]
    [Google Scholar]
  12. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria London: Cambridge University Press; 1965
    [Google Scholar]
  13. Lányi B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987;19:1–67[CrossRef]
    [Google Scholar]
  14. Hucker GJ, Conn HJ. Method of gram staining. NY State Agric Exp Stn Tech Bull 1923;93:3–37
    [Google Scholar]
  15. Schaeffer AB, Fulton MD. A simplified method of staining endospores. Science 1933;77:194 [CrossRef][PubMed]
    [Google Scholar]
  16. Ruan Z, Wang Y, Zhang C, Song J, Zhai Y et al. Clostridium huakuii sp. nov., an anaerobic, acetogenic bacterium isolated from methanogenic consortia. Int J Syst Evol Microbiol 2014;64:4027–4032 [CrossRef][PubMed]
    [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  18. Xu XW, Huo YY, Wang CS, Oren A, Cui HL et al. Pelagibacterium halotolerans gen. nov., sp. nov. and Pelagibacterium luteolum sp. nov., novel members of the family Hyphomicrobiaceae. Int J Syst Evol Microbiol 2011;61:1817–1822 [CrossRef][PubMed]
    [Google Scholar]
  19. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI, Inc; 1990
    [Google Scholar]
  20. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996;42:989–1005 [CrossRef]
    [Google Scholar]
  21. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984;25:125–128 [CrossRef]
    [Google Scholar]
  22. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  23. Tamburini E, Daly S, Steiner U, Vandini C, Mastromei G. Clostridium felsineum and Clostridium acetobutylicum are two distinct species that are phylogenetically closely related. Int J Syst Evol Microbiol 2001;51:963–966 [CrossRef][PubMed]
    [Google Scholar]
  24. Ren N, Xing D, Rittmann BE, Zhao L, Xie T et al. Microbial community structure of ethanol type fermentation in bio-hydrogen production. Environ Microbiol 2007;9:1112–1125 [CrossRef][PubMed]
    [Google Scholar]
  25. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  26. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  27. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  28. Horino H, Ito M, Tonouchi A. Clostridium oryzae sp. nov., from soil of a Japanese rice field. Int J Syst Evol Microbiol 2015;65:943–951 [CrossRef][PubMed]
    [Google Scholar]
  29. Gupta RS, Gao B. Phylogenomic analyses of clostridia and identification of novel protein signatures that are specific to the genus Clostridium sensu stricto (cluster I). Int J Syst Evol Microbiol 2009;59:285–294 [CrossRef][PubMed]
    [Google Scholar]
  30. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  31. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001702
Loading
/content/journal/ijsem/10.1099/ijsem.0.001702
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error