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Volume 67, Issue 4

sp. nov., isolated from tap water Free

Abstract

A Gram-stain-negative, strictly aerobic, rod-shaped bacterium, designated W1-2-1, was isolated from tap water in South Korea. The strain was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-1 grew at 18–42 °C and at pH 6.0–10.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus and is most closely related to the JCM 12082 (97.2 % similarity), NBRC 15499 (96.8 %), CP1D (96.8 %), NBRC 15498 (96.8 %), ODN7 (96.4 %) and DSM 14562 (95.8 %). Chemotaxonomic data [major ubiquinone – Q10, major polyamine – homospermidine, major fatty acids – summed feature 8 (Cω7/ω6), C and C 2-OH and presence of sphingoglycolipid] supported the affiliation of the strain to the genus . The G+C content of genomic DNA was 67.1 mol%. However, low level of DNA–DNA relatedness value between strain W1-2-1 and JCM 12082 and the results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain W1-2-1 from other species with validly published names. Therefore, the isolate represents a novel species, for which the name sp. nov. (type strain W1-2-1=KACC 18309=LMG 28596) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , polyphasic taxonomy , Sphingomonas aquatica and tap water
© 2017 IUMS
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2017-04-01
2024-04-19
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Sphingomonas aquatica sp. nov., isolated from tap water
Int J Syst Evol Microbiol 67, 845 (2017); https://doi.org/10.1099/ijsem.0.001682
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