1887

Abstract

A thermophilic, anaerobic, fermentative bacterium, strain A6, was obtained from an anaerobic batch digester treating animal manure and rice straw. Cells were Gram-stain-positive, slightly curved rods with a size of 0.6–1×2.5–8.2 µm, non-motile and produced terminal spores. The temperature, pH and NaCl concentration ranges for growth were 40–60 °C, 6.5–8.0 and 0–15.0 g l, with optimum growth noted at 50–55 °C, pH 7.5 and in the absence of NaCl, respectively. Yeast extract was required for growth. -Glucose, maltose, -xylose, -galactose, -fructose, -ribose, lactose, raffinose, sucrose, -arabinose, cellobiose, -mannose and yeast extract were used as carbon and energy sources. The fermentation products from glucose were ethanol, lactate, acetate, propionate, butyrate, valerate, iso-butyrate, iso-valerate, H and CO. The G+C content of the genomic DNA was 36.6 mol%. The predominant fatty acids were C, iso-C, C, C 7, C N-alcohol and C 3-OH. Respiratory quinones were not detected. The polar lipid profile comprised phosphoglycolipids, phospholipids, glycolipids, a diphosphatidylglycerol, a phosphatidylglycerol and an unidentified lipid. Phylogenetic analyses of the 16S rRNA gene sequence indicated that the strain was closely related to DSM 22681 with a similarity of 96.0 %. Based on the morphological, physiological and taxonomic characterization, strain A6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A6 (=DSM 28090=ACCC 19951).

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2017-05-01
2024-03-29
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