1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium, designated HSF11, was isolated from deep seawater of the South China Sea. Growth was observed at 20–40 °C (optimum 35 °C), pH 5.0–8.5 (optimum pH 7.0–7.5) and with 0–7 % NaCl (optimum 2 %). Bacteriochlorophyll a and poly-β-hydroxybutyrate (PHB) granules were not detected. Nitrate could be reduced to nitrite. The major fatty acids (≥5 %) of strain HSF11 were C19 : 0cyclo ω8c, C18 : 0 and C16 : 0. The isoprenoid quinone was Q-10. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unknown phospholipids (PL1, PL2), one unknown aminolipid (AL1) and three unknown lipids (L1, L3, L4). The genomic DNA G+C content was 70.7 mol%. 16S rRNA gene sequence analysis indicated that strain HSF11 was most closely related to Tepidamorphus gemmatus DSM 19345 (95.7 % 16S rRNA gene sequence similarity) and Lutibaculum baratangense KCTC 22669 (95.2 %). On the basis of the genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain HSF11 represents a novel species of a new genus, for which the name Microbaculum marinum gen. nov., sp. nov. is proposed. The type strain of the type species is HSF11 (=KCTC 52 363=MCCC 1K03192).

Keyword(s): deep seawater and Proteobacteria
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2017-05-05
2019-10-15
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