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Abstract

A Gram-stain-negative, non-spore-forming, non-motile and aerobic coccobacilli-shaped strain, designated BRTC-1, was isolated from the gut of Hampson, which is a larva of a moth and was collected from Xishuangbanna Dai Autonomous Prefecture in China. The isolate was found to grow at NaCl concentrations of 0–5 % (w/v) (optimum: 0 %), 10–45 °C (optimum: 30–35 °C) and pH 5.0–9.0 (optimum: pH 6.0) on tryptic soy agar. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus and was most closely related to LMG 26107, LMG 988 and LMG 1003 with 96.4, 96.3 and 96.3 % sequence similarity, respectively. The comparative sequence analyses of the and genes showed that BRTC-1 is distant from the species of the genus with validly published names (≤84.0 and ≤82.0 % similarity, respectively). The average nucleotide identity and digital DNA–DNA hybridization values (≤77.0 and ≤22.8 %, respectively) between the whole-genome sequence of BRTC-1 and those of the known taxa were far below the thresholds used to discriminate bacterial species. The major fatty acids were determined to be C, Cω9 and Cω7/iso-C 2-OH. The respiratory quinone was Q-9. The polar lipids were found to be diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, five phospholipids and one phosphatidylcholine. Based on its phenotypic and chemotaxonomic characteristics from this study, the isolate is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BRTC-1 (=ACCC 19936=JCM 31367).

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2017-04-01
2019-12-15
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