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Abstract

A bacterial strain, designated MIB-4, was isolated from a freshwater spring in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain MIB-4 were Gram-staining-negative, aerobic, non-motile rods that formed pale pink colonies. Growth occurred at 15–30 °C (optimum, 25 °C), at pH 7–8 (optimum, pH 7) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MIB-4 belonged to the genus and was most closely related to CL-GP79 with sequence similarity of 98.7 %. Strain MIB-4 contained Cω5, iso-C and summed feature 3 (Cω7 and/or Cω6) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, six unidentified aminophospholipids, one unidentified glycolipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content of the genomic DNA was 37.6 mol%. The DNA–DNA hybridization value for strain MIB-4 with CL-GP79 was less than 32 %. On the basis of the phylogenetic inference and phenotypic data, strain MIB-4 should be classified as a representative of a novel species, for which the name sp. nov. is proposed. The type strain is MIB-4 (=BCRC 80926=LMG 29292=KCTC 33763).

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2017-02-01
2020-12-01
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