1887

Abstract

A Gram-stain-positive, facultatively anaerobic actinomycete, designated strain T6220-5-2b, was isolated from a sample taken from a mouldy spot on the surface of a mural painting (the white tiger, ) inside the stone chamber of Takamatsuzuka Tumulus in Asuka village, Nara Prefecture, Japan. Based on 16S rRNA gene sequence analysis of the isolate, it was closely related to the genus , but formed of a novel lineage within the family . The closest related species to strain T6220-5-2b was , with which it shared 99.1 % 16S rRNA gene sequence similarity. The isoprenoid quinone systems were menaquinones MK-9(H), MK-9(H) and MK-9(H). The predominant cellular fatty acids for the isolate were anteiso-C and iso-C. The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid. The cell-wall acyl type was acetyl. The major polar lipids of the isolate were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside, two unknown phospholipids and an unknown phosphoglycolipid. Whole-cell sugars of the isolate were galactose, glucose and ribose. The DNA G+C content of the genomic DNA was 75.2 mol%. Based on the results of phylogenetic, physiological and biochemical analyses and DNA–DNA hybridization experiments, the isolate was considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is T6220-5-2b (=JCM 28789=NCIMB 15040). The reclassification of as comb. nov. is also proposed with the emended description of this species.

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2017-02-01
2024-12-08
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