@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001615, author = "El Houari, Abdelaziz and Ranchou-Peyruse, Magali and Ranchou-Peyruse, Anthony and Dakdaki, Adrien and Guignard, Marion and Idouhammou, Lahcen and Bennisse, Rhizlane and Bouterfass, Radia and Guyoneaud, Rémy and Qatibi, Abdel-Illah", title = "Desulfobulbus oligotrophicus sp. nov., a sulfate-reducing and propionate-oxidizing bacterium isolated from a municipal anaerobic sewage sludge digester", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "2", pages = "275-281", doi = "https://doi.org/10.1099/ijsem.0.001615", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001615", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "sludge digester", keywords = "sulfate reduction", keywords = "Desulfobulbus", keywords = "disproportionation of sulfite", abstract = "A novel, mesophilic, strictly anaerobic, sulfate-reducing and propionate-oxidizing bacterium, strain Prop6T, was enriched and isolated from a municipal anaerobic sewage sludge digester. Cells were Gram-stain-negative, catalase-positive, oval rods, motile by means of amphitrichous flagella, non-spore-forming and contained menaquinone MK-5(H2) as the major respiratory quinone. The genomic DNA G+C content was 51.7 mol%. The optimal NaCl concentration, temperature and pH were 2–5 g l−1, 35 °C and pH 7.6, respectively. Strain Prop6T could only oxidize propionate, lactate and pyruvate (weakly) with sulfate, sulfite or thiosulfate, mainly to acetate. Strain Prop6T fermented pyruvate and lactate to acetate and propionate. The predominant cellular fatty acids were C14 : 0, C16 : 0, C16 : 1ω7, C16 : 1ω5, C17 : 1ω6 and C18 : 1ω7. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the newly isolated strain was a member of the genus Desulfobulbus , with Desulfobulbus elongatus DSM 2908T, Desulfobulbus propionicus DSM 2032T and Desulfobulbus rhabdoformis DSM 8777T as closest relatives among species with validly published names. On the basis of genotypic, phenotypic and chemotaxonomic characteristics, it is proposed that the isolate represents a novel species, Desulfobulbus oligotrophicus sp. nov. The type strain is Prop6T (=DSM 103420T=JCM 31535T).", }