1887

Abstract

Three strains, FXJ8.095, FXJ8.057 and H201, were isolated from deep-sea hydrothermal plume water collected at the Southwest Indian Ridge at a depth of 2800 m. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates formed a closely related subcluster within the genus . The 16S rRNA gene sequence of strain FXJ8.095 shared 99.90 and 99.60 % similarity with those of strains FXJ8.057 and H201, respectively, and 98.81, 98.75, 98.68 and 98.10 % with those of ‘’ JCM 17929, HO-9041, HO-9042 and JCM 11614, respectively. DNA–DNA hybridization values among the three new isolates were higher than 70 %, while the values between each of the isolates and the closely related type strains were well below 70 %. Random amplified polymorphic DNA fingerprint patterns and a combination of physiological and biochemical properties also distinguished the isolates from the related species. The major cellular fatty acids of the isolates were anteiso-C and iso-C, the predominant menaquinones were MK-7(H) and MK-8(H), and the main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C contents of strains FXJ8.095, FXJ8.057 and H201 were 75.6, 72.8 and 70.4 mol%, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, we propose to classify the three strains in a novel species named sp. nov., with FXJ8.095 (=CGMCC 4.6946=DSM 24949) as the type strain.

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2017-01-01
2020-01-22
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