1887

Abstract

A Gram-stain-negative, orange-pigmented, non-spore-forming, non-motile, aerobic, rod-shaped bacterial strain, designated CAU 1131, was isolated from reclaimed land. Strain CAU 1131 grew optimally at 30 °C and at pH 6.5 in the presence of 4 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1131 was grouped into the genus Nonlabens , and was most closely related to Nonlabens. marinus S1-08 (95.9 % 16S rRNA gene sequence similarity). The strain possessed+ MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15:0 3-OH, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major cellular fatty acids. The polar lipid profile was determined to comprise phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid, an unidentified glycolipid and two unidentified lipids. The DNA G+C content was 38.7 mol%. On the basis of data from phenotypic, chemotaxonomic and phylogenetic inference, strain CAU 1131 represents a novel species of the genus Nonlabens , for which the name Nonlabens halophilus sp. nov. is proposed. The type strain is CAU 1131 (=KCTC 52177=NBRC 111996).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001590
2017-02-20
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/1/138.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001590&mimeType=html&fmt=ahah

References

  1. Garrity GM, Holt JG. The road map to the manual. In Boone DR, Castenholz RW, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 1 New York: Springer; 2001; pp 119– 166 [CrossRef]
    [Google Scholar]
  2. Lau SC, Tsoi MM, Li X, Plakhotnikova I, Dobretsov S et al. Nonlabens tegetincola gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a microbial mat in a subtropical estuary. Int J Syst Evol Microbiol 2005; 55: 2279– 2283 [CrossRef] [PubMed]
    [Google Scholar]
  3. Yi H, Chun J. Unification of the genera nonlabens, Persicivirga, Sandarakinotalea and Stenothermobacter into a single emended genus, Nonlabens, and description of Nonlabens agnitus sp. nov. Syst Appl Microbiol 2012; 35: 150– 155 [CrossRef] [PubMed]
    [Google Scholar]
  4. Parte AC. LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014; 42: D613– D616 [CrossRef] [PubMed]
    [Google Scholar]
  5. Yoon JH, Kang SJ, Lee CH, Oh TK. Donghaeana dokdonensis gen. nov., sp. nov., isolated from sea water. Int J Syst Evol Microbiol 2006; 56: 187– 191 [CrossRef] [PubMed]
    [Google Scholar]
  6. Nedashkovskaya OI, Kwon KK, Kim SJ. Reclassification of Donghaeana dokdonensis Yoon et al. 2006 as Persicivirga dokdonensis comb. nov. and emended descriptions of the genus Persicivirga and of Persicivirga xylanidelens O'sullivan et al. 2006. Int J Syst Evol Microbiol 2009; 59: 824– 827 [CrossRef] [PubMed]
    [Google Scholar]
  7. O'Sullivan LA, Rinna J, Humphreys G, Weightman AJ, Fry JC. Culturable phylogenetic diversity of the phylum 'Bacteroidetes' from river epilithon and coastal water and description of novel members of the family Flavobacteriaceae: Epilithonimonas tenax gen. nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov. Int J Syst Evol Microbiol 2006; 56: 169– 180 [CrossRef] [PubMed]
    [Google Scholar]
  8. Khan ST, Nakagawa Y, Harayama S. Sandarakinotalea sediminis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 2006; 56: 959– 963 [CrossRef] [PubMed]
    [Google Scholar]
  9. Lau SC, Tsoi MM, Li X, Plakhotnikova I, Dobretsov S et al. Stenothermobacter spongiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a marine sponge in the Bahamas, and emended description of Nonlabens tegetincola. Int J Syst Evol Microbiol 2006; 56: 181– 185 [CrossRef] [PubMed]
    [Google Scholar]
  10. Barbeyron T, Lerat Y, Sassi JF, Le Panse S, Helbert W et al. Persicivirga ulvanivorans sp. nov., a marine member of the family Flavobacteriaceae that degrades ulvan from green algae. Int J Syst Evol Microbiol 2011; 61: 1899– 1905 [CrossRef] [PubMed]
    [Google Scholar]
  11. Park S, Yoshizawa S, Chiura HX, Muramatsu Y, Nakagawa Y et al. Nonlabens marinus sp. nov., a novel member of the Flavobacteriaceae isolated from the Pacific Ocean. Antonie van Leeuwenhoek 2012; 102: 669– 676 [CrossRef] [PubMed]
    [Google Scholar]
  12. Park S, Kang CH, Yoon JH. Nonlabens arenilitoris sp. nov., a member of the family Flavobacteriaceae isolated from seashore sand. Antonie van Leeuwenhoek 2013; 103: 1125– 1132 [CrossRef] [PubMed]
    [Google Scholar]
  13. Kwon YM, Yang SH, Kwon KK, Kim SJ. Nonlabens antarcticus sp. nov., a psychrophilic bacterium isolated from glacier ice, and emended descriptions of Nonlabens marinus Park et al. 2012 and Nonlabens agnitus Yi and Chun 2012. Int J Syst Evol Microbiol 2014; 64: 400– 405 [CrossRef] [PubMed]
    [Google Scholar]
  14. Gordon RE, Mihm JM. Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 1962; 98: 628– 636 [CrossRef]
    [Google Scholar]
  15. Lane DJ. 16S/23S RNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics London: Wiley; 1991; pp 115– 175
    [Google Scholar]
  16. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal w and Clustal x version 2.0. Bioinformatics 2007; 23: 2947– 2948 [CrossRef] [PubMed]
    [Google Scholar]
  17. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  18. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  19. Fitch WM, Margoliash E. Construction of phylogenetic trees. Science 1967; 155: 279– 284 [PubMed] [CrossRef]
    [Google Scholar]
  20. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HH. editor Mammalian Protein Metabolism New York: Academic Press; 1969; pp 21– 132 [CrossRef]
    [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783– 791 [CrossRef]
    [Google Scholar]
  22. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25: 125– 128 [CrossRef]
    [Google Scholar]
  23. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  24. Nicholson WL, Setlow P. Sporulation, germination and outgrowth. In Harwood CR, Cutting SM. (editors) Molecular Biological Methods for Bacillus Chichester: Wiley; 1990; pp 391– 450
    [Google Scholar]
  25. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50: 1861– 1868 [CrossRef] [PubMed]
    [Google Scholar]
  26. Cappuccino JG, Sherman N. Microbiology: a Laboratory Manual, 6th edn. Menlo Park, CA: Benjamin/Cummings; 2002
    [Google Scholar]
  27. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp 607– 654
    [Google Scholar]
  28. Yoshizawa S, Kawanabe A, Ito H, Kandori H, Kogure K. Diversity and functional analysis of proteorhodopsin in marine Flavobacteria. Environ Microbiol 2012; 14: 1240– 1248 [CrossRef] [PubMed]
    [Google Scholar]
  29. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M. Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 1980; 188: 221– 233 [CrossRef]
    [Google Scholar]
  30. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19: 161– 207 [CrossRef]
    [Google Scholar]
  31. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001590
Loading
/content/journal/ijsem/10.1099/ijsem.0.001590
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error