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Abstract

A novel Gram-stain-negative, slightly halophilic, motile, curved rod with a horseshoe shape, designated strain Bsw-2b, was isolated from Badab-Soort travertine spring in Iran. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Bsw-2b belongs to the order , showing 84.6 % sequence similarity to DSM 19535 and 84.4 % and 83.9 % sequence similarity to CL-KR2 and DSM 17893, respectively. In addition, phenotypic and physiological features could clearly differentiate strain Bsw-2b from species of the most closely related genera, , , and . The strain was able to grow with 1–3 % (w/v) (optimum at 2 %) NaCl, at temperatures of 28–34 °C (optimum at 30 °C) and between pH 6.0 and 8.0 (optimum at pH 7.0). The major cellular fatty acids of strain Bsw-2b were iso-C, iso-C and iso-C. The polar lipid profile of strain Bsw-2b was composed predominantly of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and four unknown phospholipids. The DNA G+C content was 40.5 mol%. Based on the evidence from the polyphasic study, strain Bsw-2b represents a novel species in a novel genus within a new family, for which the name gen. nov., sp. nov. is proposed, within the new family fam. nov. The type strain is strain Bsw-2b (=IBRC-M 10915=LMG 28547).

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2017-01-01
2019-12-11
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