1887

Abstract

A novel Gram-stain-negative, slightly halophilic, motile, curved rod with a horseshoe shape, designated strain Bsw-2b, was isolated from Badab-Soort travertine spring in Iran. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Bsw-2b belongs to the order , showing 84.6 % sequence similarity to DSM 19535 and 84.4 % and 83.9 % sequence similarity to CL-KR2 and DSM 17893, respectively. In addition, phenotypic and physiological features could clearly differentiate strain Bsw-2b from species of the most closely related genera, , , and . The strain was able to grow with 1–3 % (w/v) (optimum at 2 %) NaCl, at temperatures of 28–34 °C (optimum at 30 °C) and between pH 6.0 and 8.0 (optimum at pH 7.0). The major cellular fatty acids of strain Bsw-2b were iso-C, iso-C and iso-C. The polar lipid profile of strain Bsw-2b was composed predominantly of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and four unknown phospholipids. The DNA G+C content was 40.5 mol%. Based on the evidence from the polyphasic study, strain Bsw-2b represents a novel species in a novel genus within a new family, for which the name gen. nov., sp. nov. is proposed, within the new family fam. nov. The type strain is strain Bsw-2b (=IBRC-M 10915=LMG 28547).

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2017-01-01
2024-03-29
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References

  1. Munoz R, Rosselló-Móra R, Amann R. Revised phylogeny of Bacteroidetes and proposal of sixteen new taxa and two new combinations including Rhodothermaeota phyl. nov. Syst Appl Microbiol 2016; 39:281–296 [View Article][PubMed]
    [Google Scholar]
  2. Wang YX, Liu JH, Xiao W, Zhang XX, Li YQ et al. Fodinibius salinus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt mine. Int J Syst Evol Microbiol 2012; 62:390–396 [View Article][PubMed]
    [Google Scholar]
  3. Wang YX, Liu JH, Xiao W, Ma XL, Lai YH et al. Aliifodinibius roseus gen. nov., sp. nov., and Aliifodinibius sediminis sp. nov., two moderately halophilic bacteria isolated from salt mine samples. Int J Syst Evol Microbiol 2013; 63:2907–2913 [View Article][PubMed]
    [Google Scholar]
  4. Xia J, Ling SK, Wang XQ, Chen GJ, du ZJ. Aliifodinibius halophilus sp. nov., a moderately halophilic member of the genus Aliifodinibius, and proposal of Balneolaceae fam. nov. Int J Syst Evol Microbiol 2016; 66:2225–2233 [View Article][PubMed]
    [Google Scholar]
  5. Urios L, Agogué H, Lesongeur F, Stackebrandt E, Lebaron P. Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea. Int J Syst Evol Microbiol 2006; 56:1883–1887 [View Article][PubMed]
    [Google Scholar]
  6. Urios L, Intertaglia L, Lesongeur F, Lebaron P. Balneola alkaliphila sp. nov., a marine bacterium isolated from the Mediterranean Sea. Int J Syst Evol Microbiol 2008; 58:1288–1291 [View Article][PubMed]
    [Google Scholar]
  7. Cho Y, Chung H, Jang GI, Choi DH, Noh JH et al. Gracilimonas rosea sp. nov., isolated from tropical seawater, and emended description of the genus Gracilimonas. Int J Syst Evol Microbiol 2013; 63:4006–4011 [View Article][PubMed]
    [Google Scholar]
  8. Pentecost A. Travertine Netherlands: Springer-Verlag Berlin Heidelberg; 2005
    [Google Scholar]
  9. Tindall BJ, Rosselló-Móra R, Busse HJ, Ludwig W, Kämpfer P. Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 2010; 60:249–266 [View Article][PubMed]
    [Google Scholar]
  10. Murray RGE, Doetsch RN, Robinow CF. Determinative and cytological light microscopy. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 21–41
    [Google Scholar]
  11. Bernardet JF, Vancanneyt M, Matte-Tailliez O, Grisez L, Tailliez P et al. Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals. Syst Appl Microbiol 2005; 28:640–660 [View Article][PubMed]
    [Google Scholar]
  12. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Kreig NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
    [Google Scholar]
  13. Harrigan WF, McCance ME. Laboratory Methods in Food and Dairy Microbiology London: Academic Press; 1976
    [Google Scholar]
  14. Ventosa A, Quesada E, Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A. Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 1982; 128:1959–1968
    [Google Scholar]
  15. Mata JA, Martínez-Cánovas J, Quesada E, Béjar V. A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 2002; 25:360–375 [View Article][PubMed]
    [Google Scholar]
  16. Quesada E, Ventosa A, Ruiz-Berraquero F, Ramos-Cormenzana A. Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 1984; 34:287–292 [CrossRef]
    [Google Scholar]
  17. Choi DH, Zhang GI, Noh JH, Kim WS, Cho BC. Gracilimonas tropica gen. nov., sp. nov., isolated from a Synechococcus culture. Int J Syst Evol Microbiol 2009; 59:1167–1172 [View Article][PubMed]
    [Google Scholar]
  18. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Molec Biol 1961; 3:208–218 [CrossRef]
    [Google Scholar]
  19. Mehrshad M, Amoozegar MA, Didari M, Bagheri M, Fazeli SA et al. Bacillus halosaccharovorans sp. nov., a moderately halophilic bacterium from a hypersaline lake. Int J Syst Evol Microbiol 2013; 63:2776–2781 [View Article][PubMed]
    [Google Scholar]
  20. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004; 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  21. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  22. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376[PubMed] [CrossRef]
    [Google Scholar]
  23. Felsenstein J. Confidence limits on phylogenies: an approach using bootstrap. Evolution 1985; 39:783–791 [CrossRef]
    [Google Scholar]
  24. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  25. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996; 42:989–1005 [CrossRef]
    [Google Scholar]
  26. Groth I, Schumann P, Weiss N, Martin K, Rainey FA. Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 1996; 46:234–239 [View Article][PubMed]
    [Google Scholar]
  27. Monciardini P, Cavaletti L, Schumann P, Rohde M, Donadio S. Conexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria. Int J Syst Evol Microbiol 2003; 53:569–576 [View Article][PubMed]
    [Google Scholar]
  28. Cashion P, Holder-Franklin MA, Mccully J, Franklin M. A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 1977; 81:461–466[PubMed] [CrossRef]
    [Google Scholar]
  29. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [CrossRef]
    [Google Scholar]
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